Inflammation Research

, Volume 58, Issue 4, pp 184–191 | Cite as

Inhibitory effect of ZPDC glycoprotein on the expression of inflammation-related cytokines through p38 MAP kinase and JNK in lipopolysaccharide-stimulated RAW 264.7 cells

  • S.-J. Lee
  • K.-T. Lim



This study was carried out to investigate the anti-inflammatory potentials of 24 kDa glycoprotein isolated from Zanthoxylum piperitum DC fruit (ZPDC glycoprotein) in lipopolysaccharide (LPS)-stimulated murine macrophage cell line (RAW 264.7 cells).

Material and Methods:

RAW 264.7 cells were treated with ZPDC glycoprotein (50–200 μg/ml) in presence of LPS (2 μg/ml). The changes of the levels of inflammation-related factors were determined by using Western blot, EMSA, and RT-PCR.


ZPDC glycoprotein has inhibitory effects on the phosphorylation of p38 mitogen-activated protein (MAP) kinase and c-Jun N-terminal kinase (JNK), on the DNA binding activity of activator protein-1 (AP-1), and on the expression of c-Jun and c-Fos in LPS-stimulated RAW 264.7 cells. Interestingly, the DNA binding activity of AP-1 was attenuated by treatment with inhibitors of p38 MAP kinase and JNK. In addition, ZPDC glycoprotein (200 μg/ml) not only diminished the production of superoxide anion, hydrogen peroxide, and nitric oxide, but also suppressed the expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and proteins (iNOS, COX-2, and MMP-9) in LPS- stimulated RAW 264.7 cells.


The present study demonstrates that ZPDC glycoprotein is a natural anti-oxidant and one of the modulators of pro-inflammatory signal transduction pathways in RAW 264.7 cells.


ZPDC glycoprotein Pro-inflammatory mediators Anti-inflammatory potentials c-Jun N-terminal kinase RAW 264.7 cells 


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Copyright information

© Birkhäuser Verlag, Basel 2009

Authors and Affiliations

  1. 1.Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK 21)Chonnam National UniversityGwangjuSouth Korea
  2. 2.Molecular Biochemistry Laboratory, Biotechnology Research InstituteChonnam National UniversityGwangjuSouth Korea

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