Advertisement

Inflammation Research

, Volume 57, Issue 10, pp 479–483 | Cite as

LPS-induced endotoxic shock does not cause early brain edema formation – An MRI study in rats

  • B. Rosengarten
  • M. Walberer
  • J. Allendoerfer
  • C. Mueller
  • N. Schwarz
  • G. Bachmann
  • T. Gerriets
Article

Abstract.

Objective and design:

Early microcirculatory failure is assumed as a key factor in the development of a septic encephalopathy. However, brain edema is also a common finding in sepsis syndromes possibly interfering with the vasoregulative mechanisms of the brain. We assessed the occurrence of brain edema in a rat model of endotoxic shock.

Material and subjects:

Eleven mechanically ventilated male CD-rats.

Treatment:

Intravenous application of 5 mg/kg LPS (n = 8) or vehicle (n = 3).

Methods:

Apparent diffusion coefficient (ADC) and T2-relaxation time (T2RT) were quantified on cerebral MRI at baseline and repeatedly for up to 3.5 h after LPS-injection. Change in blood pressure was compensated with norepinephrine. Brain water content was quantified using the wet/dry method.

Results:

All LPS-treated rats developed endotoxic shock. No significant difference in T2RT or ADC was detectable before and after LPS-injection (T2RT: baseline 60.33 ± 1.21; after 3.5 h 60.15 ± 0.59; ADC: baseline 6.86 ± 0.51; after 3.5 h 6.75 ± 0.33). Post-mortem analysis did not indicate a difference in brain water content between septic and non-septic animals.

Conclusions:

Reports of early microcirculatory failure seem not to be related to the occurrence of early (≤3.5 h) brain edema.

Keywords:

Shock Sepsis Brain edema MRI Rat Lipopolysaccharide 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Birkhaueser 2008

Authors and Affiliations

  • B. Rosengarten
    • 1
  • M. Walberer
    • 2
  • J. Allendoerfer
    • 1
  • C. Mueller
    • 2
  • N. Schwarz
    • 1
    • 2
  • G. Bachmann
    • 2
  • T. Gerriets
    • 1
    • 2
  1. 1.Department of NeurologyJustus-Liebig University of GiessenGiessenGermany
  2. 2.Experimental Neurology Research GroupKerckhoff Clinic Bad NauheimBenekestrasseGermany

Personalised recommendations