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Inflammation Research

, Volume 57, Issue 2, pp 57–64 | Cite as

Glutamine decreases intestinal nuclear factor kappa B activity and pro-inflammatory cytokine expression after traumatic brain injury in rats

  • G. Chen
  • J. Shi
  • M. Qi
  • H. Yin
  • C. Hang
Article

Abstract.

Objective:

To investigate whether glutamine supplementation modulates intestinal nuclear factor kappa B (NF-κB) activity and pro-inflammatory cytokine expression after traumatic brain injury (TBI) in rats.

Materials and methods:

Right parietal cortical contusion in male rats was made by the weight-dropping method. After trauma, the rats were randomly given chow alone or glutamine mixed chow for 5 d. Gut samples were extracted at 5 d postinjury. We measured NF-κB binding activity by electrophoretic mobility shift assay; NF-κB subunits p50 and p65 expression by immunohistochemistry; the concentrations of interleukin-1β, tumor necrosis factor-α and interleukin-6 by enzyme-linked immunosorbent assay; intestinal mucosal morphological changes by histopathological study and electron microscopy; and apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining.

Results:

Administration of glutamine following TBI could decrease NF-κB binding activity, NF-κB p65 protein expression and concentrations of pro-inflammatory cytokines in the gut. TBI-induced damage of gut structure was ameliorated after glutamine supplementation.

Conclusion:

The results of the present study suggest that the therapeutic benefit of post-TBI glutamine supplementation might be due to its inhibitory effects on intestinal NF-κB activation and pro-inflammatory cytokine expression.

Keywords:

Traumatic brain injury Intestine Nuclear factor kappa B Inflammation Glutamine 

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Copyright information

© Birkhaueser 2008

Authors and Affiliations

  1. 1.Department of Neurosurgery, Jinling Hospital, School of MedicineNanjing UniversityNanjingP.R. China

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