Advertisement

Inflammation Research

, Volume 56, Issue 7, pp 282–290 | Cite as

Signal transduction pathways affected by nitric oxide donors during neutrophil functional response in vitro

  • M. Klink
  • K. Bednarska
  • K. Jastrzembska
  • M. Banasik
  • Z. Sulowska
Article

Abstract.

Objective and design:

We investigated the intracellular signalling pathways by which nitric oxide (NO) donors: diethylamine/NO (DEA/NO) and 3-morpholinosydnonimine (SIN-1) regulate the functional response of human neutrophils to activating stimuli.

Methods:

The phosphorylation and nitration of signalling proteins, cyclic GMP level, neutrophil respiratory burst and adhesive activities and CD11b/CD18 molecule expression on neutrophils in the presence and absence of soluble guanylate cyclase inhibitors were determined.

Results:

NO donors showed strong inhibitory effect on activated neutrophils. NO donors nitrated the tyrosine residues in signalling proteins causing a decrease in tyrosine phosphorylation and neutrophils response to activation. Diethylamine/NO employed cyclic GMP as a signalling molecule in its action on neutrophils, whereas peroxynitrite anion donor affected neutrophil functions in a cGMP-independent manner. Moreover, we observed that peroxynitrite anion can overcome the nitric oxide molecule action.

Conclusions:

We conclude that each NO donor depending on its concentration and chemical nature may act on different elements of neutrophil signalling pathways capable of inducing distinct neutrophil functions.

Keywords:

Nitric oxide donors Neutrophils cGMP Proteins nitration and phosphorylation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Birkhäuser Verlag, Basel 2007

Authors and Affiliations

  • M. Klink
    • 1
  • K. Bednarska
    • 1
  • K. Jastrzembska
    • 1
  • M. Banasik
    • 2
  • Z. Sulowska
    • 1
  1. 1.Centre for Medical BiologyPolish Academy of SciencesLodzPoland
  2. 2.Department of Clinical ImmunologyInstitute of Polish Mother’s Memorial HospitalLodzPoland

Personalised recommendations