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Inflammation Research

, Volume 56, Issue 6, pp 254–261 | Cite as

Potential role for epidermal Langerhans cells in nicotinic acid-induced vasodilatation in the mouse

  • E. Carballo-Jane
  • T. Ciecko
  • S. Luell
  • J. W. Woods
  • E. I. Zycband
  • M. G. Waters
  • M. J. Forrest
Original Research Paper

Abstract.

Objective:

Our objective is to study the role of cutaneous Langerhans cells on a mouse model of nicotinic acid-induced vasodilatation.

Methods:

Nicotinic acid-induced vasodilatation was studied in the mouse ear by laser Doppler flowmetry prior to and at intervals after Langerhans cells depletion by treatment with hydrocortisone.

Results:

Nicotinic acid evoked a dose-dependent increase in perfusion in the mouse ear. Treatment with 1 % hydrocortisone resulted in substantial depletion of Langerhans cells, accompanied by failure to show vasodilatation in response to nicotinic acid. Partial recovery of Langerhans cells on day 53 post-treatment was associated with a partial vasodilatation response. To exclude non-specific effects of hydrocortisone on arachidonic acid metabolism, the ability of the mice to mount an edema response to phorbol 12-myristate 13-acetate was evaluated. On day 9 post hydrocortisone, phorbol 12-myristate 13-acetate failed to evoke an edema response. However, on day 22 post hydrocortisone, the edema response in the hydrocortisone-treated animals was indistinguishable from that of control animals.

Conclusions:

These results suggest that Langerhans cells are responsible for nicotinic acid-induced vasodilatation.

Keywords:

Nicotinic acid Langerhans cells Vasodilatation Mouse model 

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Copyright information

© Birkhäuser Verlag, Basel 2007

Authors and Affiliations

  • E. Carballo-Jane
    • 1
  • T. Ciecko
    • 1
  • S. Luell
    • 1
  • J. W. Woods
    • 2
  • E. I. Zycband
    • 2
  • M. G. Waters
    • 3
  • M. J. Forrest
    • 1
  1. 1.Department of PharmacologyMerck Research Laboratories, RahwayRahwayUSA
  2. 2.Department of ImmunologyMerck Research LaboratoriesRahwayUSA
  3. 3.Department of Cardiovascular DiseasesMerck Research Laboratories RahwayRahwayUSA

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