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Inflammation Research

, Volume 55, Issue 9, pp 364–367 | Cite as

Fasudil, a Rho-kinase inhibitor, inhibits leukocyte adhesion in inflamed large blood vessels in vivo

  • J. E. Slotta
  • O. Ö. Braun
  • M. D. Menger
  • H. Thorlacius
Original Paper

Abstract.

Objective and Design

Emerging data suggest that Rho-kinase signaling may regulate numerous aspects of inflammatory reactions. Herein, we investigated the role of Rho-kinase in inflammatory interactions between leukocytes and the endothelium in femoral arteries and veins in vivo.

Material and methods

Mice were injected with lipopolysaccharide (LPS) and Rho-kinase was inhibited by pre-treatment with fasudil, which is a highly selective inhibitor of Rho-kinase. Six hours after LPS challenge, intravital fluorescence microscopy of the femoral vessels was performed and leukocyte-endothelium interactions were visualized after in vivo staining with rhodamine 6G.

Results

LPS increased leukocyte rolling and adhesion in femoral arteries and veins. Pre-treatment with fasudil had no effect on leukocyte rolling but significantly decreased venular leukocyte adhesion by 85% and completely abrogated leukocyte adhesion in femoral arteries in endotoxin-treated mice.

Conclusions

We conclude that Rho-kinase signaling regulates LPS-induced leukocyte adhesion in femoral arteries and veins in vivo and that inhibition of Rho-kinase may be useful in the treatment of pathological inflammation in large blood vessels of the vascular system.

Key words.

Adhesion Inflammation Leukocyte Microcirculation Sepsis 

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Copyright information

© Birkhäuser Verlag, Basel 2006

Authors and Affiliations

  • J. E. Slotta
    • 1
    • 2
  • O. Ö. Braun
    • 1
  • M. D. Menger
    • 2
  • H. Thorlacius
    • 1
  1. 1.Department of Surgery, Malmö University HospitalLund UniversityMalmöSweden
  2. 2.Institute for Clinical and Experimental SurgeryUniversity of SaarlandHomburgGermany

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