Inflammation Research

, Volume 53, Issue 6, pp 245–252 | Cite as

Activation of histamine H1-receptor enhances neurotrophic factor secretion from cultured astrocytes

  • M. Lipnik-Štangelj
  • M. Čarman-Kržan
Original Paper


Objective:Histamine stimulates nerve growth factor (NGF) secretion from cultured astrocytes. Histamine H1-receptor antagonists completely block its effect. In the present study, we determined the involvement of histamine-receptor subtypes in this process.

Materials and methods:Radioligand-binding assay was used to establish the presence of histamine H1- and H2-receptors on new-born rat cortical astrocytes in primary culture. Histamine H1-, H2- and H3/H4-receptor ligands, and highly selective protein kinase C (PKC) inhibitor were used to influence NGF secretion from cultured astrocytes. NGF, released into the culture medium, was measured by NGF-ELISA.

Results:Histamine H1-receptor agonists (histamine, selected histaprodifens) increased the secretion of NGF from cultured astrocytes in a concentration-dependent manner. H1-receptor antagonists/inverse agonists (mepyramine, triprolidine) and PKC inhibitor completely blocked the effect of histamine. Histamine H2- and H3-receptor agonists did not enhance NGF secretion significantly. In addition, H2- and H3/H4-receptor antagonists did not diminish histamine-stimulated NGF release.

Conclusions:Our results indicate that histamine H1-receptor and PKC are involved in the signal transduction pathway, responsible for histamine-stimulated NGF secretion from cultured astrocytes.

Histamine Histamine H1-receptor Histamine H2-receptor Histamine H3-receptor Histamine H4-receptor NGF Astrocytes 


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Copyright information

© Birkhäuser-Verlag Basel 2004

Authors and Affiliations

  1. 1.Department of PharmacologyFaculty of MedicineLjubljanaSlovenia

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