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Inflammation Research

, Volume 53, Issue 4, pp 150–157 | Cite as

Dual inhibition of 5-lipoxygenase/cyclooxygenase by a reconstituted homeopathic remedy; possible explanation for clinical efficacy and favourable gastrointestinal tolerability

  • R. Jäggi
  • U. Würgler
  • F. Grandjean
  • M. Weiser
Original Paper

Abstract

Objective: In order to elucidate potential anti-inflammatory activities of Zeel comp. N and its constituents, the inhibition of the synthesis of Leukotriene B4 (LTB4) and Prostaglandin (PGE2) by 5-lipoxygenase (5-LOX) and cyclo-oxygenase 1 and 2 (COX 1 and 2) respectively were examined in vitro.

Materials: Human HL-60 cells, differentiated for 6–8 days with DMSO (1.2% v/v) were used for the 5-LOX assay. The COX activity assays were carried out with purified enzymes, COX 1 (ram seminal vesicles), COX 2 (sheep placenta) and with human THP-1 cells, differentiated for 24 h with PMA (50 nM).

Methods: LTB4 and PGE2 production in the 5-LOX and COX assays respectively were determined by enzyme linked immunoassays.

Results: A reconstituted Zeel comp. N combination as well as its constituent mother tinctures of Arnica montana, Sanguinaria canadensis and Rhus toxicodendron (Toxicodendron quercifolium) showed distinct inhibitory effects on the production of LTB4 by 5-LOX (IC50 values of 10, 20, 2 and 5 µg/ml respectively) and on the synthesis of PGE2 by COX 1 (IC50 values of 50, 80, 40 and 20 µg/ml respectively) and COX 2 enzymes (IC50 values of 60, 110, 50 and 20 µg/ml respectively). The mother tincture of Solanum dulcamara inhibited the production of PGE2 by COX 1 (IC50 40 µg/ml) and COX 2 (IC50 150 µg/ml) but not production of leukotriene LTB4 by 5-LOX.

Conclusions: The observed dual inhibition of both LOX- and COX-metabolic pathways may offer an explanation for the reported clinical efficacy and the favorable gastrointestinal tolerability of the original remedy Zeel comp. N.

Zeel comp. N mother tinctures in vitro assays dual 5-LOX/COX inhibition 

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Copyright information

© Birkhäuser-Verlag Basel 2004

Authors and Affiliations

  • R. Jäggi
    • 1
  • U. Würgler
    • 1
  • F. Grandjean
    • 1
  • M. Weiser
    • 2
  1. 1.VitaPlant AGWitterswilSwitzerland
  2. 2.Institute for Antihomotoxic Medicine and Matrix ResearchBaden-BadenGermany

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