Immunohistochemical localization of metallothionein and p53 protein in pancreatic serous cystadenomas
- 308 Downloads
The objective of this study was to determine the expression levels of metallothionein (MT) and p53 protein, recognized neoplastic transformation markers, in pancreatic serous cystadenomas (SCA) and adenomocarcinomas.
Materials and Methods
Neoplastic pancreatic tissue was taken from 20 patients with diagnosed benign (SCA: 5 cases) or malignant tumors (adenomocarcinomas: 15 cases) and control pancreatic tissue from healthy persons who had died in car accidents. Sections were stained with hematoxylin-eosin. Immunohistochemical localization of MT and p53 protein was carried out by LSAB2-HRP using specific antibodies against MT and p53.
Metallothionein expression was observed only in the epithelial cells of the neoplastic tissue of SCAs. MT expression in the cystadenomas was weaker than in the healthy pancreatic tissue. No tissue was found with p53 protein expression. In the adenomocarcinomas, positive staining for MT was observed in 67% and p53 was positive in the carcinoma cells.
The weak MT expression and lack of p53 protein expression in pancreatic SCAs confirms the lack of local invasive potential of the neoplastic lesion. Increased expressions of MT and p53 were observed in the less differentiated tumors. Thus the expression of MT may be a potential prognostic marker for tumors.
Keywordsserous cystadenoma adenomocarcinoma pancreas immunochemistry metallothionein p53 protein
- Alrefaie W, Katz MH, Easter DW et al (2004) Simultaneous serous cystadenoma of the pancreas and mucinous cystadenoma of the appendix. J Pancreas 5: 97–100Google Scholar
- Joseph MG, Banerjee D, Kocha W et al (2001) Metallothionein expression in patients with small cell carcinoma of the lung. Correlation with other molecular markers and clinical outcome. Cancer 92: 836–842Google Scholar
- Klöppel G, Solcia E, Longnecker DS et al (1996) Histological typing of tumours of the exocrine pancreas, second edition.. Springer-Verlag, Berlin-Heidelberg-New York, pp 7–18Google Scholar
- Madej JA, Milnerowicz H, Dzimira S (1999) Immunohistochemical localization of metallothionein (MT) in epithelial tumors in animals. Med Wet 55: 806–808Google Scholar
- Milnerowicz H, Sliwinska-Mosson M, Bizon A et al (2006) Expression of metallothionein and p53 in pancreatic tumors in relation to prognosis. Gut 55: A324Google Scholar