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Evaluation of lectin pathway activity and mannan-binding lectin levels in the course of pregnancy complicated by diabetes type 1, based on the genetic background

  • Magdalena Pertyńska−Marczewska
  • Maciej Cedzyński
  • Anna Świerzko
  • Agnieszka Szala
  • Małgorzata Sobczak
  • Katarzyna Cypryk
  • Jan Wilczyński
Open Access
Original Article

Abstract

Introduction

There are numerous indications that either mannan-binding lectin (MBL) deficiency or its excessive activity are associated with adverse pregnancy outcomes. High MBL concentrations and corresponding MBL2 genotypes were shown to be associated with microvascular complications in type 1 diabetes. The aim of this study was to evaluate levels of MBL and MBL-dependent activity of the lectin pathway (LP) of complement in the course of pregnancy in diabetic mothers, based on genetic background.

Materials and Methods

These parameters were determined in samples from healthy non-pregnant (control), diabetic non-pregnant, healthy pregnant, and pregnant diabetic women.

Results

No significant differences in median MBL levels or LP activities were found in any study group compared to the control. However, statistically significant differences in MBL levels were noted during pregnancy between the 1st and 3rd trimesters in both healthy controls and pregnant diabetics. With regard to LP values, similar trends were evident, but statistically significant results were obtained only in the healthy pregnant group. When data analysis was confined to patients carrying the A/A (wild-type) MBL2 genotype, an increase in MBL level during pregnancy (in both healthy and diabetic pregnant women) was still observed. Similarly, LP activity increased during both healthy and diabetic pregnancies, significantly so for the former.

Conclusions

Diabetes, an autoimmune disease, is a serious complication of pregnancy. Therefore, determination of MBL status might be beneficial in identifying type 1 diabetic patients who are at increased risk of developing both vascular complications and poor pregnancy outcomes.

Keywords

mannan-binding lectin lectin pathway activity diabetes pregnancy 

Abbreviations

MBL

mannan-binding lectin

LP

lectin pathway

SNPs

single-nucleotide polymorphisms

PD

diabetic pregnant

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Copyright information

© L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland 2009

Authors and Affiliations

  • Magdalena Pertyńska−Marczewska
    • 1
  • Maciej Cedzyński
    • 2
  • Anna Świerzko
    • 2
  • Agnieszka Szala
    • 2
  • Małgorzata Sobczak
    • 3
  • Katarzyna Cypryk
    • 4
  • Jan Wilczyński
    • 1
  1. 1.Department of Fetal-Maternal Medicine and GynecologyResearch Institute Polish Mother’s Memorial HospitalŁódźPoland
  2. 2.Laboratory of Immunobiology of Infections, Institute of Medical BiologyPolish Academy of SciencesŁódźPoland
  3. 3.Specialized Outpatient Clinic, Department of Fetal-Maternal Medicine and GynecologyResearch Institute Polish Mother’s Memorial HospitalŁódźPoland
  4. 4.Department of Diabetology and Metabolic DiseasesMedical University of ŁódźŁódźPoland

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