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Biofilm-formation by drug-resistant Staphylococcus aureus from cow milk

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Abstract

The present study aims to quantify biofilm formation and determine the drug resistance profile of Staphylococcus aureus isolated from raw, pasteurized and ultra-high temperature (UHT) milk. Detection of S. aureus was made in 90 cow milk samples (30 raw, 30 pasteurized and 30 UHT). Biofilm biomass quantification was performed in 96-well polystyrene plates using the crystal violet technique. The resistance profile was determined by the disc-diffusion test. S. aureus was detected in 24.4% (22/90) of milk samples, with the highest prevalence in pasteurized milk. Biofilm production was observed in 100% of the S. aureus isolates from pasteurized and UHT milk, and in 81.8% of the strains from raw milk. Biofilm production by S. aureus drug-resistant strains was detected in 46.7% (14/30) of the isolates. In order to mitigate this problem, it is recommended to apply appropriate hygiene practices along the milk production chain.

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Authors and Affiliations

  1. INTA University Center - UNINTA, Sobral, 62050-100, Brazil

    Renata Albuquerque Costa, Jeniffer Vasconcelos de Lira & Márcia Facundo Aragão

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  1. Renata Albuquerque Costa
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  2. Jeniffer Vasconcelos de Lira
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  3. Márcia Facundo Aragão
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Correspondence to Renata Albuquerque Costa.

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Costa, R.A., de Lira, J.V. & Aragão, M.F. Biofilm-formation by drug-resistant Staphylococcus aureus from cow milk. J Consum Prot Food Saf 14, 63–69 (2019). https://doi.org/10.1007/s00003-018-1186-5

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  • DOI: https://doi.org/10.1007/s00003-018-1186-5

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