Abstract
The aim of this work was to evaluate the performance of spontaneous and Lactobacillus cultures in Spanish style fermentation of olive fruits. A fermentation with Lactobacillus plantarum ATCC 8014 was carried out for comparison. Fermentation was performed at room temperature during 120 days in two different concentrations of NaCl (2.4–13.6 % w/v) and sucrose (0–0.82 % w/v) in the brines. The pH, chlorides, lactic acid, reducing sugar and lactic bacteria in selective medium were investigated during the olive fermentation. After 120 days of fermentation, the formulation with 12 % NaCl in the brine hampered the growth of lactic bacteria, avoiding the consumption of reducing sugars. However, low salt concentrations (≤4 %) promoted a decrease in pH, an increase of the lactic acid and the growth of lactic bacteria, providing best environmentally suitable for the fermentation process. Whereas using concentrations of 12 % NaCl and 0.7 % sucrose showed better chemical conditions, ensuring the safety of the product produced. Multivariate analysis showed that the olives with both cultures were in close relation to lactic acid and reducing sugar and different from lactic bacteria.
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References
American Public Health Association (APHA) (2001) Compendium of methods for the microbiological examination of foods, 4th edn. American Public Health Association, Washington, DC, p 1219
Aponte M, Ventorino V, Blaiotta G, Volpe G, Farina V, Avellone G, Moschetti G (2010) Study of green Sicilian table olive fermentations through microbiological, chemical and sensory analyses. Food Microbiol. doi:10.1016/j.fm.2009.09.010
Bautista-Gallego J, Arroyo-López FN, Durán-Quintana MC, Garrido-Fernández A (2010) Fermentation profiles of Manzanilla-Aloreña cracked green table olives in different chloride salt mixtures. Food Microbiol. doi:10.1016/j.fm.2009.11.015
Bautista-Gallego J, Arroyo-López FN, Romero Gil V, Rodríguez Gómez F, García García P, Garrido-Fernández A (2011) Chloride salt mixtures affect Gordal cv. green Spanish-style table olive fermentation. Food Microbiol 28:1316–1325
Borcakli M, Özay G, Alperden I (1995) Fermentation of black olives with application of starter culture and aeration. Dev Food Sci 37:1849–1863
Castro A, Montaño A, Casado F-J, Rejano L (2002) Utilization of Enterococcus casseli flavus and Lactobacillus pentosus as starter cultures for Spanish-style green olive fermentation. Food Microbiol 19:637–644
Chammem N, Kachouri M, Mejri M, Peres C, Boudabous A, Hamdi M (2005) Combined effect of alkali pretreatment and sodium chloride addition on the olive fermentation process. Biores Technol. doi:10.1016/j.biortech.2004.10.005
Chorianopoulos NG, Boziaris IS, Stamatiou A, Nychas GJE (2005) Microbial association and acidity development of unheated and pasteurized green-table olives fermented using glucose or sucrose supplements at various levels. Food Microbiol. doi:10.1016/j.fm.2004.04.010
Codex Alimentarius (1981) Norma del Codex para las aceitunas de mesa. Standard for table olives. Stan. 66:1–18
Consejo Oleícola Internacional—IOC (2004) Norma Comercial aplicable a lãs aceitunas de Mesa—Resolución 2/91-IV/04. http://www.internationaloliveoil.org/. Accessed 07 Nov
Corsetti A, Perpetuini G, Schirone M, Tofalo R, Suzzi G (2012) Application of starter cultures to table olive fermentation: an overview on the experimental studies. Front Microbiol. doi:10.3389/fmicb.2012.00248
Cunha SC, Ferreira IMPLO, Fernandes JO, Faria MA, Oliveira PPB, Ferreira MA (2001) Determination of lactic, acetic, succinic, and citric acids in table olives by HPLC/UV. J Liquid Chromat Relat Technol 24:1029–1038
Durán Quintana MC, Romero Barranco C, García García P, Brenes Balbuena M, Garrido Fernández A (1997) Bacterias del ácido láctico en la fermentación de aceitunas de mesa. Grasas Aceites 48:297–311
Durán Quintana MC, García García P, Garrido Fernánez A (1999) Establishment of conditions for green table olive fermentation at low temperature. Int J Food Microbiol 51:135–143
Garrido-Fernández A, Díez MJF, Adams MR (1997) Table olives: production and processing. Chapman and Hall, London 499
Hurtado A, Reguant C, Bordons A, Rozès N (2009) Influence of fruit ripeness and salt concentration on the microbial processing of Arbequina table olives. Food Microbiol. doi:10.1016/j.fm.2009.05.010
Hurtado A, Reguant C, Bordons A, Rozès N (2012) Lactic acid bacteria from fermented table olives. Food Microbiol. doi:10.1016/j.fm.2012.01.006
Hutkins RW (2006) Microbiology and technology of fermented foods. Blackwell Publishing, London
Jimenez-Diaz R, Rios-Sanchez RM, Desmazeaud M, Ruiz-Barba JL, Piard JC (1993) Plantaricins S and T, two new bacteriocins produced by Lactobacillus plantarum LPCO10 isolated from a green olive fermentation. Appl Environ Microbiol 59:1416–1424
Kailis S, Harris D (2007) Producing table olives. Australia, Landlinks, p 344
Kiai H, Hafidi A (2014) Chemical composition changes in four green olive cultivars during spontaneous fermentation. Food Sci Technol 57:663–670. doi:10.1016/j.lwt.2014.02.011
Maldonado MB, Zuritz CA, Assof MV (2008) Diffusion of glucose and sodium chloride in green olives during curing as affected by lye treatment. J Food Eng. doi:10.1016/j.jfoodeng.2007.04.033
Marsilio V, Lanza B, Pozzi N (1996) Progress in table olive debittering: degradationin vitro of oleuropein and its derivatives by Lactobacillus plantarum. J Am Oil Chem Soc. doi:10.1007/BF02518113
Marsilio V, Campestre C, Lanza B, Angelis M (2001) Sugar and polyol compositions of some European olive fruit varieties (Olea europaea L.) suitable for table olive purposes. Food Chem. doi:10.1016/S0308-8146(00)00268-5
Martorana A, Alfonzo A, Settanni L, Corona O, La Croce F, Caruso T, Moschetti G, Francesca N (2015) An innovative method to produce green table olives based on “pied de cuve” technology. Food Microbiol 50:126–140
Martorana A, Alfonzo A, Settanni L, Corona O, La Croce F, Caruso T, Moschetti G, Francesca N (2016) Effect of the mechanical harvest of drupes on the quality characteristics of green fermented table olives. J Sci Food Agric 96:2004–2017
Medina E, Romero C, Castro A, Brenes M, García A (2008) Inhibitors of lactic acid fermentation in Spanish-style green olive brines of the Manzanilla variety. Food Chem. doi:10.1016/j.foodchem.2008.02.084
Miller GL (1959) Use of dinitrosalicyclic reagent for determination of reducing sugar. Analy Chem. doi:10.1021/ac60147a030
Montaño A, Sánchez AH, Casado FJ, Castro A, Rejano L (2003) Chemical profile of industrially fermented green olives of different varieties. Food Chem. doi:10.1016/S0308-8146(02)00593-9
Özay G, Borcakli M (1995) Effect of brine replacement and salt concentration on the fermentation of naturally black olives. Food Res Int. doi:10.1016/0963-9969(95)00054-2
Panagou EZ, Schillinger U, Franz CMAP, Nychas GJE (2008) Microbiological and biochemical profile of cv. Conservolea naturally black olives during controlled fermentation with selected strains of lactic acid bacteria. Food Microbiol. doi:10.1016/j.fm.2007.10.005
Poiana M, Romeo FV (2006) Changes in chemical and microbiological parameters of some varieties of Sicily olives during natural fermentation. Grasas Aceites. doi:10.3989/gya.2006.v57.i4.66
Randazzo CL, Fava G, Tomaselli F, Romeo FV, Pennino G, Vitello E, Caggia C (2011) Effect of kaolin and copper based products and of starter cultures on green table olive fermentation. Food Microbiol. doi:10.1016/j.fm.2010.12.004
Rodríguez H, Curiel JA, Landete JM, Rivas B, Felipe FL, Gómez-Cordovés C, Muñoz R (2009) Food phenolics and lactic acid bacteria. Int J Food Microbiol. doi:10.1016/j.ijfoodmicro.2009.03.025
Romeo FV, Piscopo A, Poiana M (2010) Effect of acidification and salt concentration on two black brined olives from Sicily (c v moresca and giarraffa). Grasas Aceites 61:251–260
Romeo FV, Piscopo A, Mincione A, Poiana M (2012) Quality evaluation of different typical table olive preparations (cv Nocellara del Belice). Grasas Aceites 61:19–25
Ruiz-Barba JL, Jiménez-Díaz R (2012) A novel Lactobacillus pentosus-paired starter culture for Spanish-style green olive fermentation. Food Microbiol. doi:10.1016/j.fm.2011.11.004
Tassou CC, Panagou EZE, Katsaboxakis KZ (2002) Microbiological and physicochemical changes of naturally black olives fermented at different temperatures and NaCl levels in the brines. Food Microbiol 19:605–615
Tsapatsaris S, Kotzekidou P (2004) Application of central composite design and response surface methodology to the fermentation of olive juice by Lactobacillus plantarum and Debaryomyces hansenii. Int J Food Microbiol. doi:10.1016/j.ijfoodmicro.2004.02.011
Zago M, Lanza B, Rossetti L, Muzzalupo I, Carminati D, Giraffa G (2013) Selection of Lactobacillus plantarum strains to use as starters in fermented table olives: oleuropeinase activity and phage sensitivity. Food Microbiol. doi:10.1016/j.fm.2012.11.005
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The authors thank CNPq, CAPES, FAPERGS, Federal Institute Catarinense, Science and Technology Secretary RS, EMBRAPA Temperate Climate—RS and URI-Erechim for the financial support for this research.
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Rosa, A.D., da Silveira, S.M., Coutinho, E.F. et al. Green olive fermentation using spontaneous and Lactobacillus plantarum cultures. J. Verbr. Lebensm. 11, 249–257 (2016). https://doi.org/10.1007/s00003-016-1034-4
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DOI: https://doi.org/10.1007/s00003-016-1034-4