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In vitro tuberization in white yam (Dioscorea rotundata Poir)

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Abstract

Nodal cuttings of white yam were induced to produce microtubers on a MS-revised medium supplemented with various concentrations of sucrose, 20 mgl−1 L-cysteine, 0.5 mgl−1 kinetin and 0.7% agar. The frequency of tuberization was affected by the daylength, which is optimal at 12 and 16 h of light depending on the sucrose concentration. The microtubers were planted in a seed bed and grown to maturity. The importance of in vitro tuberization of yam as a means of international germplasm distribution or exchange as well as for the propagation of planting material is discussed.

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References

  1. Abbot A J, Belcher A R (1986) Potato tuber formation in vitro. In: Withers L A, Alderson P G (Eds) Plant Tissue Culture and its Agricultural Applications. pp 113–122. Butterworths

  2. Ammirato P V (1984) Yams. In: Ammirato P V, Evans D A, Sharp W R, Yamada Y (Eds) Handbook of Plant Cell Culture. Vol. 3, Crop Species, pp 327–354. MacMillan, New York

    Google Scholar 

  3. Asnani V L, Otoo J A, Hahn S K (1985) Improved Seed Yam Production Technology. p. 10. International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria

    Google Scholar 

  4. Chandler F L, Haque S Q (1984) The use of tissue culture in the production of improved yam and sweet potato planting material. In: Micropropagation of Selected Root Crops, Palms, Citrus and Ornamentals. Proceedings FAO/Norway Symposium on plant tissue culture, technology and utilization. pp 68–87. Food and Agriculture Organization of the United Nations, Rome

    Google Scholar 

  5. Estrada R, Tover P, Dodds J H (1986) Induction of in vitro tubers in a broad range of potato genotypes. Plant Cell Tissue Org Cult 7 (1): 3–10

    Google Scholar 

  6. FAO (1986) FAO production yearbook, 1985. 39. Food and Agricultural Organization of the United Nations, Rome

    Google Scholar 

  7. Forsyth C, VanStaden J (1984) Tuberization of Dioscorea bulbifera stem nodes in culture. J Pl Physiol 115: 79–83

    Google Scholar 

  8. IITA (1984) Annual Report for 1983. pp 133–134. IITA, Ibadan, Nigeria

    Google Scholar 

  9. Krul W R, Colclasure G C (1977) Effect of galactose and other monosaccharides on IAA movement in bean hypocotyl segment. Physiol Plant 41: 249–253

    Google Scholar 

  10. Mantell S H, Haque S Q, Whitehall A P (1979) A rapid propagation system for yams. Yam Virus Project Bulletin No 1, p 18. Carribean Agricultural Research and Development Institute, St. Augustine, Trinidad

    Google Scholar 

  11. Mantell S H, Haque S Q, Whitehall A P (1980) Apical meristem tip culture for eradication of flexous rod viruses in yam (D. alata). Tropical Pest Management 26 (2): 170–179

    Google Scholar 

  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15: 473–497

    Google Scholar 

  13. Ng SYC (1985) Recommendations for Handling Tissue Culture Materials (sweet potato, yam and cassava). Manual Series No 6, p 13. IITA, Ibadan, Nigeria

    Google Scholar 

  14. Rossel H W, Thottappilly G (1982) Latest research results on root and tuber crop viruses and their indexing. Paper presented at Workshop for Tropical Root and Tuber Crops Germplasm Distribution and Plant Quarantine Regulations, 28–30 April, 1982. IITA, Ibadan, Nigeria

    Google Scholar 

  15. Sengupta J, Mitra G C, Sharma A K (1984) Organogenesis and tuberization in cultures of Dioscorea floribunda. Plant Cell Tissue Org Cult 3 (4): 325–331

    Google Scholar 

  16. Uduebo A E (1971) Effect of external supply of growth substances on axillary proliferation and development in Dioscorea bulbifera. Ann Bot 35: 159–163

    Google Scholar 

  17. Wang P J, Hu C Y (1984) In vitro mass tuberization and virus-free seed-potato production in Taiwan. Am Pot J 59: 33–37

    Google Scholar 

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Ng, S.Y.C. In vitro tuberization in white yam (Dioscorea rotundata Poir). Plant Cell Tiss Organ Cult 14, 121–128 (1988). https://doi.org/10.1007/BF00041185

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  • DOI: https://doi.org/10.1007/BF00041185

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