Russian Journal of Genetics

, Volume 41, Issue 1, pp 60–65 | Cite as

RAPD and ISSR analyses of regenerated pea Pisum sativum l. plants

  • O. I. Kuznetsova
  • O. A. Ash
  • G. A. Hartina
  • S. A. Gostimskij
Plant Genetics


Long-term pea callus cultures of different genotypes (mutants R-9 and W-1 and cultivar Viola) were used to regenerate plants (generation R 0 ). The regenerants displayed changes both in qualitative and in quantitative traits. The most dramatic morphological alterations and complete sterility were observed in regenerants of the cultivar Viola. To estimate the genetic differences, regenerants were compared with the original lines with the use of RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeat) analyses. The extent of divergence varied among regenerants and depended mostly on the original genotype. The genetic difference from the original line was no more than 1% in W-1 regenerants, 0.7–5.3% in R-9 regenerants, and 10– 15% in sterile regenerants of the cultivar Viola. The genetic variation of plants regenerated from a callus culture maintained for ten years did not exceed that of plants obtained from a culture maintained for two years.


Genetic Variation Quantitative Trait Sequence Repeat Genetic Difference Callus Culture 
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Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • O. I. Kuznetsova
    • 1
  • O. A. Ash
    • 1
  • G. A. Hartina
    • 1
  • S. A. Gostimskij
    • 1
  1. 1.Department of GeneticsMoscow State UniversityMoscowRussia

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