Abstract
Methemoglobin (metHb) was used as a mimetic enzyme for peroxidase to catalyze the oxidation reaction of o-phenylenediamine (OPDA) with H2O2 functioning as an oxidant. A reaction intermediate was obtained in two-phase aqueous-organic system and an absorption peak at 710 nm was confirmed to be that of the intermediate in relation to OPDA. The isolated product and intermediate were characterized by UV-V is and IR spectrophotometry and HPLC-tandem mass spectrometry. The results showed that the product is 2,3-diaminophenazine, the molecular mass of the intermediate is 212 daltons, and a conceivable structure of the intermediate is suggested. Combining the catalyzed reaction mechanism of peroxidase and our experimental results, a conceivable oxidation reaction mechanism of OPDA and H2O2 using metHb as catalyst is proposed.
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Abbreviations
- OPDA:
-
o-phenylenediamine
- Hb:
-
hemoglobin
- metHb:
-
methemoglobin
- HRP:
-
horseradish peroxidase
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Translated from Biokhimiya, Vol. 70, No. 1, 2005, pp. 113–122. Original Russian Text Copyright © 2005 by De-Jia Li, Xi-Wen Li, Yu-Xiang Xie, Xiao-Qiang Cai, Guo-Lin Zou. Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-101, August 29, 2004.
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Li, DJ., Li, XW., Xie, YX. et al. Identification of intermediate and product from methemoglobin-catalyzed oxidation of o-phenylenediamine in two-phase aqueous—organic system. Biochemistry (Moscow) 70, 92–99 (2005). https://doi.org/10.1007/PL00021766
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DOI: https://doi.org/10.1007/PL00021766