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Hydroxyurea induces a senescence-like change of K562 human erythroleukemia cell

  • Joo-In Park
  • Jin-Sook Jeong
  • Jin-Yeong Han
  • Deok-In Kim
  • Yan-Hua Gao
  • Sang-Chul Park
  • Griffin P. Rodgers
  • In-Hoo Kim
ORIGINAL ARTICLE

Abstract

Hydroxyurea, a differentiation-inducing agent of human erythroleukemia K562 cells, is commonly used to treat some types of leukemia. However, the mechanism for its therapeutic effect is not clearly understood yet. In this study, we have observed an interesting effect of hydroxyurea on tumor cells: an induction of senescence-like changes. Human erythroleukemia K562 cells, when treated with hydroxyurea for 7 days or more, underwent a change into phenotypically senescent cells together with a reduction of hemoglobin generation, a differentiation marker. The hydroxyurea-treated cells showed positive senescence associated-β-galactosidase staining, a senescence index, and the accumulation of cdk (cyclin dependent kinase) inhibitors, such as p16INK4a, p21Waf1, and p27Kip1, implicated in cellular senescence. Nonetheless, these changes were not accompanied by DNA fragmentation. Taken together, we summarize that the long-term treatment of cancer cells with hydroxyurea can induce cellular senescence different from differentiation or programmed cell death.

Key words Hydroxyurea Senescence Erythroleukemia K562 cells 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Joo-In Park
    • 1
  • Jin-Sook Jeong
    • 2
  • Jin-Yeong Han
    • 3
  • Deok-In Kim
    • 1
  • Yan-Hua Gao
    • 1
  • Sang-Chul Park
    • 4
  • Griffin P. Rodgers
    • 5
  • In-Hoo Kim
    • 1
  1. 1.Department of Biochemistry, Dong-A University College of Medicine, Pusan, KoreaKR
  2. 2.Department of Pathology, Dong-A University College of Medicine, Pusan, KoreaKR
  3. 3.Department of Clinical Pathology, Dong-A University College of Medicine, Pusan, KoreaKR
  4. 4.WHO Collaborating Centre of Physical Culture and Aging Research for Health Promotion, Seoul National University College of Medicine, Seoul, KoreaKR
  5. 5.Molecular and Clinical Hematology Branch, NIDDK/NIH, Bethesda, Maryland, USAUS

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