Biochemical Genetics

, Volume 28, Issue 5–6, pp 233–246 | Cite as

The fatty acid constitution and ordering state of membranes in dominant temperature-sensitive lethal mutation and wild-typeDrosophila melanogaster larvae

  • Janos Szidonya
  • Tibor Farkas
  • Tibor Pali


The ordering state and changes in fatty acid composition of microsomal (MS) and mitochondrial (MC) membranes of two dominant temperature-sensitive (DTS) lethal mutations and the wild-type Oregon-R strain larvae ofDrosophila melanogaster have been studied at 18 and 29°C and after temperature-shift experiments. The membranes of wild-type larvae have a stable ordering state, with “S” values between 0.6 (18°C) and 0.5 (29°C) in both membranes which remained unchanged in shift experiments, although the ratios of saturated/unsaturated fatty acids were changed as expected. The stronglyDTS mutation1(2)10DTS forms very rigid membranes at the restrictive temperature (29°C) which cannot be normalized after shift down, while shift up or development at the permissive temperature results in normal ordering state. This mutant is less able to adjust MS and MC fatty acid composition in response to the growth temperature than the wild type. The less temperature-sensitive1(2)2DTS allele occupies an intermediate state between Oregon-R and1(2)10DTS in both respects. We assume and the genetical data suggest that the DTS mutant gene product is in competition with the wild-type product, resulting in a membrane structure which is not able to accommodate to the restrictive temperature.

Key words

Drosophila melanogaster membranes dominant temperature sensitive wild type fatty acids spin label 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chapman, E., Wright, L. C., and Raison, J. K. (1979). Seasonal changes in the structure and function of mitochondrial membranes of artichoke tubers a requisite for surviving low temperatures during dormancy.Plant Physiol. 63363.Google Scholar
  2. Cossins, A. R. (1977). Adaptation of biological membranes to temperature. The effect of temperature acclimation of goldfish upon viscosity of synaptosomal membranes.Biochim. Biophys. Acta 470395.Google Scholar
  3. Cossins, A. R., and Prosser, C. L. (1978). Evolutionary adaptation of membranes to temperature.Proc. Natl. Acad. Sci. USA 752040.Google Scholar
  4. Dickens, B. F., and Thompson, G. A., Jr. (1981). Rapid membrane response during low temperature acclimation. Correlation of early changes in the physical properties and lipid composition of Tetrahymena microsomal membrane.Biochim. Biophys. Acta 64456.Google Scholar
  5. Farkas, T. (1984). Adaptation of fatty acid composition to temperature-A study on carp (Cyprinus carpio) liver slices.Comp. Biochem. Physiol. 79B531.Google Scholar
  6. Farkas, T., Nemecz, Gy., and Csengeri, I. (1984). Differential response of lipid metabolism and physical state by an actively and passively overwintering planktonic crustacean.Lipids 19436.Google Scholar
  7. Folch, J., Lees, M., and Sloane-Stanley, G. H. (1957). A siple method for the isolation of total lipids from animal tissues.J. Biol. Chem. 226497.Google Scholar
  8. Furtado, D., Williams, W. P., Brain, A. P. R., and Quinn, P. J. (1979). Phase separation in membranes ofAnacystis nidulans grown at different temperatures.Biochim. Biophys. Acta 555352.Google Scholar
  9. Griffith, O. H., and Jost, P. C. (1976). In Berliner, L. J. (ed.),Spin Labeling. Theory and Applications Academic Press, New York, p. 478.Google Scholar
  10. Holden, J. J., and Ashburner, M. (1978). Patterns of puffing activity in the salivary gland chromosomes ofDrosophila. IX. The salivary and prothoracic gland chromosomes of a dominant temperature sensitive lethal ofDrosophila melangaster.Chromosoma 68205.Google Scholar
  11. Janoff, A. S., Haug, A., and McGroarty, E. J. (1979). Relationship of growth temperature and thermotrophic lipid phase changes in cytoplasmic and outer membranes fromEscherichia coli K 12.Biochim. Biophys. Acta 55556.Google Scholar
  12. Janoff, A. S., Gupte, S., and McGroarty, E. (1980). Correlation between temperature range of growth and structural transitions in membranes and lipids ofEscherichia coli K 12.Biochim. Biophys. Acta 598:641.J.Google Scholar
  13. Kallapur, V. L., Downer, R. G., George, J. C., and Thompson, J. E. (1982). Effect of environmental temperature on the phase properties and lipid composition of fligh muscle mitochondria ofSchistocera gregaria.Insect Biochem. 12115.Google Scholar
  14. Lindsley, D., and Zimm, G. (1986). The genome ofDrosophila melanogaster.Drosoph. Inform. Serv. 6446.Google Scholar
  15. Martin, Ch. E., Siegel, D., and Aaronson, L. R. (1981). Effects of temperature on neurospora phospholipid fatty acid desaturation appears to be a key element in modifying phospholipid fluid properties.Biochim. Biophys. Acta 665399.Google Scholar
  16. Ramesha, C. S., and Thompson, G. A., Jr. (1982). Changes in the lipid composition and physical properties of Tetrahymena ciliary membranes following low-temperature acclimation.Biochemistry 213612.Google Scholar
  17. Reuter, G., and Szidonya, J. (1983). Cytogenetic analysis of variegation suppressors and dominant temperature-sensitive lethal in region 23–26 of chromosome 2L inDrosophila melanogaster.Chromosoma 88277.Google Scholar
  18. Rosenbluth, R., Ezzel, D., and Suzuki, D. T. (1971). Temperature-sensitive mutations inDrosophila melanogaster. IX. Dominant cold-sensitive lethals on the authosomes.Genetics 7075.Google Scholar
  19. Sinenski, M. (1974). Homeoviscous adaptation. A homeostatic process that regulates the viscosity of membrane lipids inEscherichia coli K 12.Proc. Natl. Acad. Sci. USA 71522.Google Scholar
  20. Suzuki, D. T., and Procunier, D. (1969). Temperature-sensitive mutations inDrosophila melanogaster. III. Dominant lethals and semilethals on chromosome 2.Proc. Natl. Acad. Sci. USA 62369.Google Scholar
  21. Szidonya, J., and Reuter, G. (1988). Cytogenetic analysis of theechinoid (ed),dumpy (dp) andclot (cl) region inDrosophila melanogaster.Genetic. Res. Cambr. 51197.Google Scholar
  22. Umeki, S., Maruyama, H., and Nozawa, Y. (1983). Studies on thermal adaptation of Tetrahymena lipids. Alteration in fatty acid composition and its mechanism in the growth temperature shift up.Biochim. Biophys. Acta 75230.Google Scholar
  23. Vigh, L., Horvath, I., Farkas, T., Horvath, L. I., and Belea, A. (1979). Adaptation of membrane fluidity of rye and wheat seedlings according to temperature.Phytochemistry 18787.Google Scholar
  24. Vigh, L., Horvath, I., Woltjes, J., Farkas, T., van Hasselt, P., and Kuiper, P. J. C. (1986). Combined electron-spin-resonance, X-ray-diffraction studies on phospholipid vesicles obtained from cold-hardened wheats. I. An attempt to correlate electron-spin-resonance spectral characteristics with frost tolerance.Planta 16914.Google Scholar

Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Janos Szidonya
    • 1
  • Tibor Farkas
    • 2
  • Tibor Pali
    • 3
  1. 1.Institute of Genetics, Biological Research CenterHungarian Academy of SciencesSzegedHungary
  2. 2.Institute of Biochemistry, Biological Research CenterHungarian Academy of SciencesSzegedHungary
  3. 3.Institute of Biophysics, Biological Research CenterHungarian Academy of SciencesSzegedHungary

Personalised recommendations