Abstract
Phenotypic diversity of self-incompatibility (S) alleles within nine natural populations ofLycopersicon peruvianum was investigated. Only 7 incompatible responses were observed of a total of 276 unique combinations tested, on the basis of controlled pollinations, indicating the large number of alleles that exist within these populations. Molecular weight polymorphism for specific major stylar proteins observed on SDS-PAGE was also evident in two of the populations examined. Five proteins were shown to map to theS locus and to be associated with differentS alleles through controlled pollinations and segregation of the proteins. Two of theseS related proteins had been described previously in terms of spatial and temporal expression consistent with their involvement in self-incompatibility (Mauet al., Planta 169, 184–191, 1986). A mapping population derived from a fully compatible cross was used to establish linkage of theS locus to two DNA markers,CD15 andTG184, that lie on chromosome 1. The order of the markers and estimates of map distances are given.
Similar content being viewed by others
References
Anderson, M. A., Cornish, E. C., Mau, S.-L., Williams, E. G., Hoggart, R., Atkinson, A., Bonig, I., Grego, B., Simpson, R., Roche, P. J., Haley, J. D., Pennschow, J. D., Niall, H. D., Tregear, G. W., Coghlan, J. P., Crawford, R. J., and Clarke, A. E. (1986). Cloning of a cDNA for a style glycoprotein associated with expression of self-incompatibility inNicotiana alata.Nature 32138.
Anderson, M. A., McFadden, G. I., Bernatzky, R., Atkinson, A., Orpin, T., Dedman, H., Tregear, G., Fernley, R., and Clarke, A. E. (1989). Sequence variability of three alleles of the self-incompatibility gene ofNicotiana alata.Plant Cell 1483.
Atwood, S. S. (1944). Oppositional alleles in natural populations ofTrifolium repens.Genetics 29428.
Bernatzky, R., and Schilling, A. (1992). Methods for Southern blotting and hybridization. In Osborn, T. C., and Beckmann, J. S. (eds.),Plant Genomes: Methods for Genetic and Physical Mapping Kluwer, The Netherlands.
Bernatzky, R., and Tanksley, S. D. (1986a). Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences.Genetics 112887.
Bernatzky, R., and Tanksley, S. D. (1986b). Genetics of actin-related sequences in tomato.Theor. Appl. Genet. 72314.
Campbell, J. M., and Lawrence, M. J. (1981). The population genetics of the self-incompatibility polymorphism inPapaver rhoeas. I. The number and distribution ofS-alleles in families from three localities.Heredity 4669.
Clark, K. R., Okuley, J. J., Collins, P. D., and Sims, T. L. (1990). Sequence variability and developmental expression ofS-alleles in self-incompatible and pseudo-selfcompatible petunia.Plant Cell 2815.
de Nettancourt, D. (1977).Incompatibility in Angiosperms Springer-Verlag, New York.
Emerson, S. (1939). A preliminary survey of theOenethera organesis population.Genetics 24538.
Emerson, S. (1940). Growth of incompatible pollen tubes inOenethera organensis.Bot. Gaz. 101890.
Jahnen, W., Batterham, M. P., Clarke, A. E., Moritz, R. L., and Simpson, R. J. (1989). Identification, isolation, and N-terminal sequencing of style glycoproteins associated with self-incompatibility inNicotiana alata.Plant Cell 1493.
Laemmli, U. L. (1970). Cleavage of structural proteins during assembly of the head of bacteriophage T4.Nature 227680.
Leach, C. R. (1988). Detection and estimation of linkage for a co-dominant structural gene locus linked to a gametophytic self-incompatibility locus.Theor. Appl. Genet. 75882.
Mau, S. L., Williams, E. G., Atkinson, A., Anderson, M. A., Cornish, E. C., Greco, B., Simpson, R. J., Kheyr-Pour, A., and Clarke, A. E. (1986). Style proteins of wild tomato (Lycopersicon peruvianum) associated with expression of self-incompatibility.Planta 169184.
Miller, J. C., and Tanksley, S. D. (1990). RFLP analysis of phyogenetic relationships and genetic variation in the genusLycopersicon.Theor. Appl. Genet. 80437.
Rick, C. M. (1986). Reproductive isolation in theLycopersicon peruvianum complex. In D'arcy, W. G. (ed.),Solanaceae Biology and Systematics Columbia University Press, New York, pp. 477–495.
Suiter, K. A., Wendel, J. F., and Case, J. S. (1983). LINKAGE-1: A pascal computer program for the detection and analysis of genetic linkage.J. Hered. 74203.
Tanksley, S. D., and Loaiza-Figueroa, F. (1985). Gametophytic self-incompatibility is controlled by a single major locus on chromosome 1 inLycopersicon peruvianum.Proc. Natl. Acad. Sci. USA 825093.
Tanksley, S. D., Ganal, M. W., Prince, J. P., de Vicente, Bonierbale, M. W., Broun, P., Fulton, T. M., Giovanonni, Grandillo, S., Martin, G. B., Messeguer, R., Miller, J. C., Miller, L., Paterson, A. H., Pineda, O., Röder, M. S., Wing, R. A., Wu, W., and Young, N. D. (1992). High density molecular linkage maps of the tomato and potato genomes: Biological inferences and practical applications.Genetics 1321141–1160.
Woodward, J. R., Bacic, A., Jahnen, W., and Clarke, A. E. (1989). N-Linked glycan chains onS-allele-associated glycoproteins fromNicotiana alata.Plant Cell 1511.
Xu, B., Mu, J., Nevins, D. L., Grun, P., and Kao, T. (1990). Cloning and sequencing of cDNAs encoding two self-incompatibility associated proteins inSolanum chacoense.Mol. Gen. Genet. 224341.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bernatzky, R. Genetic mapping and protein product diversity of the self-incompatibility locus in wild tomato (Lycopersicon peruvianum). Biochem Genet 31, 173–184 (1993). https://doi.org/10.1007/PL00020537
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/PL00020537