Advertisement

Biochemical Genetics

, Volume 30, Issue 5–6, pp 279–287 | Cite as

Variation in coding exons of two electrophoretic alleles at the pigtail macaque carbonic anhydrase I locus as determined by direct, double-stranded sequencing of polymerase chain reaction (PCR) products

  • Nils C. H. Bergenhem
  • Patrick J. Venta
  • Penelope J. Hopkins
  • Richard E. Tashian
Article
  • 12 Downloads

Abstract

Two, electrophoretically distinct, forms of carbonic anhydrase I (CA Ia and CA Ib) are found at high polymorphic frequencies in red cells of natural populations of pigtail macaques,Macaca nemestrina, from southeast Asia. By use of the polymerase chain reaction, exons of the CA I gene were amplified from homozygous (a/a, b/b) and heterozygous (a/b) animals. Direct sequencing of the amplified DNA from four animals revealed differences between the a and the b electrophoretic alleles ranging from three to six nucleotides, and from one to three differences within each allele. These results indicate a greater genetic variability at the CA I locus in this macaque species than previously realized.

Key words

Macaca nemestrina carbonic anhydrase I locus polymerase chain reaction DNA sequencing allelic variation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arai, K., Madison, J., Huss, K., Ishioka, N., Satoh, C., Fujita, M., Neel, J. V., Sakurabayashi, I., and Putnam, F. W. (1989). Point substitutions in Japanese alloalbumins.Proc. Natl. Acad. Sci. USA 866092.PubMedGoogle Scholar
  2. Bachmann, B., Luke, W., and Hunsmann, G. (1990). Improvement of PCR amplified DNA sequencing with the aid of detergents.Nucleic Acids Res. 181309.PubMedGoogle Scholar
  3. Casanova, J.-L., Pannetier, C., Jaulin, C., and Kourilsky, P. (1990). Optimal conditions for directly sequencing double-stranded PCR products with Sequenace.Nucleic Acids Res. 184028.PubMedGoogle Scholar
  4. Darga, L. L., Goodman, M., Weiss, M. L., Moore, G. W., Prychodko, W., Dene, H., Tashian, R., and Koen, A. (1975). Molecular systematics and clinal variation in macaques. In Markert, C. L. (ed.),Isozymes: Genetics and Evolution, Vol. 4 Academic Press, New York, pp. 797–812.Google Scholar
  5. DeSimone, J., Magid, E., and Tashian, R. E. (1973). Genetic variation in the carbonic anhydrase isozymes of macaque monkeys. II. Inheritance of red cell carbonic anhydrase levels in different carbonic anhydrase I genotypes of the pig-tailed macque,Macaca nemestrina, Biochem. Genet. 8165.PubMedGoogle Scholar
  6. Fooden, J. (1975). Taxonomy and evolution of liontail and pigtail macaques.Fieldiana Zool. 671.Google Scholar
  7. Fraser, P. J., and Curtis, P. J. (1986). Molecular evolution of the carbonic anhydrase genes: Calculation of divergence time for mouse carbonic anhydrase II and III.J. Mol. Evol. 23294.PubMedGoogle Scholar
  8. Hewett-Emmett, D., and Tashian, R. E. (1991). Structure and evolutionary origins of the carbonic anhydrase multigene family. In Dodgson, S. J., Tashian, R. E., Gros, G., and Carter, N. D. (eds.),The Carbonic Anhydrases: Cellular Physiology and Molecular Genetics Plenum Press, New York, pp. 15–32.Google Scholar
  9. Jabusch, J. R., Bray, R. P., and Deutsch, H. F. (1980). Sequence of the low activity equine erythrocyte carbonic anhydrase and delineation of the amino acid substitutions in various polymorphic forms.J. Biol. Chem. 2559196.PubMedGoogle Scholar
  10. Kafatos, F. C., Efstratiadis, A., Forget, B. G., and Weissman, S. M. (1977). Molecular evolution of human and rabbit β-globin mRNAs.Proc. Natl. Acad. Sci. USA 745618.PubMedGoogle Scholar
  11. Konialis, C. P., Barlow, J. H., and Butterworth, P. H. W. (1985). Cloned cDNA for rabbit erythrocyte carbonic anhydrase I: A novel erythrocyte specific probe to study development in erythroid tissues.Proc. Natl. Acad. Sci. USA 82663.PubMedGoogle Scholar
  12. Lowe, N., Hugh, J. M. B., Barlow, J. H., Sowden, J. C., Edwards, M., and Butterworth, P. H. W. (1990). Structure and methylation patterns of the gene encoding human carbonic anhydrase I.Gene 93277.PubMedGoogle Scholar
  13. Neel, J. V. (1984). A revised estimate of the amount of genetic variation in human proteins. Implications for the distribution of DNA polymorphisms.Am. J. Hum. Genet. 361135.PubMedGoogle Scholar
  14. Nicewander, P. H. (1990).Sequence and Organization of a Macaca nemestrinaCarbonic Anhydrase I Gene, Ph.D. thesis, University of Michigan, Ann Arbor.Google Scholar
  15. Sandberg, K. (1968). Genetic polymorphism in carbonic anhydrase from horse erythrocytes.Hereditas 60411.Google Scholar
  16. Takahashi, N., Neel, J. V., Satoh, C., Nishizaki, J., and Masunari, N. (1982). A phylogeny for the principal alleles of the human phosphoglucomutase-1 locus.Proc. Natl. Acad. Sci. USA 796636.PubMedGoogle Scholar
  17. Tashian, R. E. (1965). Genetic variation and evolution of the carboxylic esterases and carbonic anhydrases of primate erythrocytes.Am. J. Hum. Genet. 17257.PubMedGoogle Scholar
  18. Tashian, R. E., and Carter, N. D. (1976). Biochemical genetics of carbonic anhydrase. In Harris, H., and Hirschhorn, K. (eds.),Advances in Human Genetics Plenum Press, New York, pp. 1–56.Google Scholar
  19. Tashian, R. E., Goodman, M., Headings, V. E., DeSimone, J., and Ward, R. H. (1971). Genetic variation and evolution in the red cell carbonic anhydrase isozymes of macaque monkeys.Biochem. Genet. 5183.PubMedGoogle Scholar
  20. Tashian, R. E., Hewett-Emmett, D., and Goodman, M. (1983). On the evolution and genetics of carbonic anhydrases I, II, and III. In Rattazzi, M. C., Scandalios, J. G., and Whitt, G. S. (eds.),Isozymes: Current Topics in Biological and Medical Research A. R. Liss, New York, pp. 79–100.Google Scholar
  21. Tashian, R. E., Venta, P. J., Nicewander, P. H., and Hewett-Emmett, D. (1990). Evolution, structure, and expression of the carbonic anhydrase multigene family.Prog. Clin. Biol. Res. 344159.PubMedGoogle Scholar
  22. Wagner, L. E., Venta, P. J., and Tashian, R. E. (1991). A human carbonic anhydrase I deficiency appears to be caused by a destabilizing amino acid substitution (246-Arg → His).Isozyme Bull. 2435.Google Scholar

Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Nils C. H. Bergenhem
    • 1
  • Patrick J. Venta
    • 1
    • 2
  • Penelope J. Hopkins
    • 1
  • Richard E. Tashian
    • 1
  1. 1.Department of Human GeneticsUniversity of Michigan Medical SchoolAnn Arbor
  2. 2.Small Animal Clinical Sciences, College of Veterinary MedicineMichigan State UniversityEast Lansing

Personalised recommendations