Biochemical Genetics

, Volume 30, Issue 5–6, pp 279–287 | Cite as

Variation in coding exons of two electrophoretic alleles at the pigtail macaque carbonic anhydrase I locus as determined by direct, double-stranded sequencing of polymerase chain reaction (PCR) products

  • Nils C. H. Bergenhem
  • Patrick J. Venta
  • Penelope J. Hopkins
  • Richard E. Tashian


Two, electrophoretically distinct, forms of carbonic anhydrase I (CA Ia and CA Ib) are found at high polymorphic frequencies in red cells of natural populations of pigtail macaques,Macaca nemestrina, from southeast Asia. By use of the polymerase chain reaction, exons of the CA I gene were amplified from homozygous (a/a, b/b) and heterozygous (a/b) animals. Direct sequencing of the amplified DNA from four animals revealed differences between the a and the b electrophoretic alleles ranging from three to six nucleotides, and from one to three differences within each allele. These results indicate a greater genetic variability at the CA I locus in this macaque species than previously realized.

Key words

Macaca nemestrina carbonic anhydrase I locus polymerase chain reaction DNA sequencing allelic variation 


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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Nils C. H. Bergenhem
    • 1
  • Patrick J. Venta
    • 1
    • 2
  • Penelope J. Hopkins
    • 1
  • Richard E. Tashian
    • 1
  1. 1.Department of Human GeneticsUniversity of Michigan Medical SchoolAnn Arbor
  2. 2.Small Animal Clinical Sciences, College of Veterinary MedicineMichigan State UniversityEast Lansing

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