Research in Experimental Medicine

, Volume 190, Issue 1, pp 153–162 | Cite as

Effect of medium-chain triglycerides (MCT) as an energy substrate after hepatectomy in rats with streptozotocin-induced diabetes

  • K. Mitsuyoshi
  • Y. Hiramatsu
  • M. Nakagawa
  • M. Yamamura
  • K. Hioki
  • M. Yamamoto


The suitability of energy substrates used by the remnant liver after a 70% partial hepatectomy was studied in relation to the hepatic energy status in diabetic rats. Rats with streptozotocin-induced diabetes mellitus underwent 70% hepatectomy, and were divided into three groups receiving total parenteral nutrition (TPN) for 24h. One group received standard TPN without fat, while two other groups received standard TPN with medium-chain triglycerides (MCTs) or long-chain triglycerides (LCTs) as a 10% lipid emulsion. All rats of the TPN group without fat died within 24 h. Blood ketone body ratios (acetoacetate/β-hydroxybutyrate), energy charge levels of the remnant liver, and cumulative amounts of 14CO2 in the expired breath during the 6 h following 14C-labeled fat emulsion administration (MCT or LCT) were all significantly higher in the MCT group than in the LCT group 24 h after hepatectomy. These findings suggest that medium-chain fatty acids (MCFAs) as an energy substrate are superior to long-chain fatty acids (LCFAs) during the immediate posthepatectomy phase in diabetics.

Key words

Fat emulsion Triglycerides Diabetes mellitus Hepatectomy (70%) Energy charge Rat 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Atkinson DE (1970) Enzymes as control elements in metabolic regulation. In: Boyer PD (ed) The enzymes, 1st edn. Academic Press, New York, pp 461–489Google Scholar
  2. 2.
    Bremer J, Wojtczak AB (1972) Factors controlling the rate of fatty acid β-oxidation in rat liver mitochondria. Biochim Biophys Acta 280:515–530PubMedGoogle Scholar
  3. 3.
    Crozier G, Bois BJ, Chanez M, Girard J, Peret J (1987) Metabolic effects induced by longterm feeding of medium-chain triglycerides in the rat. Metabolism 35:807–814CrossRefGoogle Scholar
  4. 4.
    French TJ, Goode AW, Schofield PS, Sugden MC (1985) Control of tissue carnitine contents: effects of partial hepatectomy and liver regeneration on carnitine concentrations in liver and extrahepatic tissues of the rat. Biosci Rep 5:47–55PubMedCrossRefGoogle Scholar
  5. 5.
    Gamble MS, Cook GA (1985) Alteration of the apparent Ki of carnitine palmitoyltransferase for malonyl-coa by the diabetic state and reversal by insulin. J Biol Chem 260: 9516–9519PubMedGoogle Scholar
  6. 6.
    Herbert V, Lau KS, Gottlieb CW, Bleicher SJ (1965) Coated charcoal immunoassay of insulin. J Clin Endocrinol 25:1375–1384CrossRefGoogle Scholar
  7. 7.
    Higgins GM, Anderson RM (1931) Experimental pathology of the liver 1. Restoration of the liver of the white rat following partial surgical removal. Arch Pathol Lab Med 12:186–202Google Scholar
  8. 8.
    Hultman E (1959) Rapid specific method for determination of aldosaccharides in body fluids. Nature 183:108–109PubMedCrossRefGoogle Scholar
  9. 9.
    Ida T, Ozawa K, Honjo I (1975) Glucose intolerance after massive liver regeneration in man and other mammals. Am J Surg 129:523–527PubMedCrossRefGoogle Scholar
  10. 10.
    Jaworek D, Gruber W, Bergmeyer HU (1974) Adenosine 5-diphosphate and adenosine 5-monophosphate. In: Bergmeyer HU (ed) Method of enzymatic analysis. Academic Press, New York, pp 2127–2131Google Scholar
  11. 11.
    Lamprecht W, Trautshold I (1974) Determination with hexokinase and glucose-6-phosphate dehydrogenase. In: Bergmeyer HU (ed) Method of enzymatic analysis. Academic Press, New York, pp 2101–2110Google Scholar
  12. 12.
    Lassow WJ, Brown CW Jr, Chaikoff IL (1956) Action of insulin in sparing fatty acid oxidation: A study with palmitic acid-1-C14 and octanoate-1-C14. J Biol Chem 220:839–849Google Scholar
  13. 13.
    Latipaa PM, Karki TT, Hiltunen JK, Hassinen IE (1986) Regulation of palmitoylcarnitine oxidation in isolated rat liver mitochondria. Role of the redox state of NADH, Biochim Biophys Acta 875:293–300PubMedGoogle Scholar
  14. 14.
    Lumeng L, Bremer J, Davis EJ (1976) Suppression of the mitochondrial oxidation of (−)-palmitoylcarnitine by the malateasparate and α-glycerolphosphate shuttles. J Biol Chem 251:277–284PubMedGoogle Scholar
  15. 15.
    Mellanby J, Williamson DH (1974) Acetoacetate. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp. 1840–1843Google Scholar
  16. 16.
    McCalla C, Gates HS Jr, Gordon RS Jr (1957) 4CO2 Excretion after the intravenous administration of albmin-bound palmitate-1-14C to intact rats. Arch Biochem 71:346–351PubMedCrossRefGoogle Scholar
  17. 17.
    McDermott WV, Ottinger LW (1966) Elective hepatic resection. Am J Surg 112:376–381PubMedCrossRefGoogle Scholar
  18. 18.
    Nakatani T, Ozawa K, Asano M, Ukikusa M, Kamiyama Y, Tobe T (1981) Differences in predominant energy substrate in relation to the resected hepatic mass in the phase immediately after hepatectomy. J Lab Clin Med 97:887–898PubMedGoogle Scholar
  19. 19.
    Ochsner JL, Meyers BE, Ochsner A (1971) Hepatic lobectomy. Am J Surg 121:273–282PubMedCrossRefGoogle Scholar
  20. 20.
    Otto DA (1984) Relationship of the ATP/ADP ratio to the site of octanoate activation. J Biol Chem 259:5490–5492PubMedGoogle Scholar
  21. 21.
    Ozawa K, Ida T, Yamada T, Yamada T, Honjo I (1976) Significance of glucose tolerance as prognostic sign in heptatectomized patients. Am J Surg 131:541–546PubMedCrossRefGoogle Scholar
  22. 22.
    Roehrig KL, Allred JB (1974) Direct enzymatic procedure for the determination of liver glycogen. Anal Biochem 58:414–421PubMedCrossRefGoogle Scholar
  23. 23.
    Ukikusa M, Osawa K, Shimaharu Y, Asano M, Nakatani T, Tobe T (1981) Changes in blood ketone body ratio. Their significance after major hepatic resection. Arch Surg 116: 781–785PubMedGoogle Scholar
  24. 24.
    Veech RL, Raijman L, Krebs HA (1970) Equilibrium relations between the cytoplasmic adenine nucleotide system and nicotinamide-adenine nucleotide system in rat liver. Biochem J 117:499–503PubMedGoogle Scholar
  25. 25.
    Williamson DH, Mellanby J (1974) d-(−)-β-hydroxybutyrate. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 1836–1839Google Scholar
  26. 26.
    Zaleski J, Ontko JA (1985) Reciprocal effects of energy utilization on palmitate oxidation and esterification in hepatocytes of fed rats. Biochim Biophys Acta 836:134–142PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • K. Mitsuyoshi
    • 1
  • Y. Hiramatsu
    • 1
  • M. Nakagawa
    • 1
  • M. Yamamura
    • 1
  • K. Hioki
    • 1
  • M. Yamamoto
    • 1
  1. 1.Department of SurgeryKansai Medical UniversityMoriguchi, OsakaJapan

Personalised recommendations