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Journal of Plant Research

, Volume 114, Issue 4, pp 509–515 | Cite as

Immunocytochemical Localization of Enzymes Involved in Lignification of the Cell Wall

  • Keiji Takabe
  • Miyuki Takeuchi
  • Takahiko Sato
  • Masaki Ito
  • Minoru Fujita
JPR Symposium

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-methyltransferase, and cinnnamyl alcohol dehydrogenase were localized to differentiating xylem. These enzymes are particularly abundant during secondary wall formation. Immunolabeling was observed on polysomes and in the cytosol of the cells during secondary wall formation, indicating that these enzymes are synthesized in the polysomes and released in the cytosol. The synthesis of monolignols might occur in the cytosol. Immunolabeling of anionic peroxidase was also localized to the differentiating xylem, particularly during secondary wall formation. The labeling, however, was observed in the rough endoplasmic reticulum (r-ER), the Golgi apparatus, and the plasma membrane, indicating that peroxidase is synthesized in the r-ER, transported to the Golgi apparatus, and localized on the plasma membrane by fusion of the Golgi vesicles to the membrane.

Keywords: Antibody, Differentiating xylem, Immunocytochemistry, Lignification, Monolignol, Poplar 

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Copyright information

© The Botanical Society of Japan 2001

Authors and Affiliations

  • Keiji Takabe
    • 1
  • Miyuki Takeuchi
    • 1
  • Takahiko Sato
    • 1
  • Masaki Ito
    • 2
  • Minoru Fujita
    • 1
  1. 1.Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto, 606–8502 JapanJP
  2. 2.Yatsushiro Mill, Nippon Paper Industries, Yatsushiro, Kumamoto, 866–0823 JapanJP

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