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Journal of Plant Research

, Volume 112, Issue 2, pp 175–186 | Cite as

Transformation of trans-Golgi Network During the Cell Cycle in a Green Alga, Botryococcus braunii

  • Tetsuko Noguchi
  • Fukiko Kakami

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-Golgi network (TGN), and the changes in its structure and behavior throughout the cell cycle of a unicellular green alga, Botryococcus braunii, were examined with deep-etching replicas and in cryo-fixed/freeze-substituted specimens. In interphase cells, the TGN consisted of a hemispherically shaped cisterna (TGN-cisterna) with regularly distributed pores on the surface and a tubular network (TGN-tubules) with clathrin-coated vesicles. The TGNs changed their structure drastically throughout the cell cycle. The TGN-cisterna disappeared from the beginning of nuclear division to the completion of the cell wall, in contrast that TGN-tubules with the clathrin-coated vesicles were always observed. The TGN-tubules produced at least five other kinds of vesicles depending on the stage of the cell cycle: 200-nm vesicles with fibrillar substances and multivesicular bodies in interphase, 180–240 nm vesicles during cell division, and 400–450 nm vesicles containing fibrils and small masses of electron-dense substances, and 200-nm vesicles containing electron-dense spherical substances just after cell division. During cell wall formation, TGN-tubules were small and had only a few clathrin-coated vesicles. After cell wall formation, TGN-tubules grew and a TGN-cisterna with pores appeared again.

Keywords: Botryococcus, Cell cycle, Coated vesicle, Golgi apparatus, trans-Golgi network 

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Copyright information

© The Botanical Society of Japan 1999

Authors and Affiliations

  • Tetsuko Noguchi
    • 1
  • Fukiko Kakami
    • 1
  1. 1.Department of Biological Science, Nara Women's University, Nara, 630–8506 JapanJP

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