Advertisement

Journal of General Plant Pathology

, Volume 66, Issue 2, pp 138–143 | Cite as

Isolation of a Protein Bound to Canker-forming Factor from Citrus Plant

  • Alfredo Gryciuk ALMEIDA
  • Kazutoshi SAYAMA
  • Shinji TSUYUMU

Interaction analysis using affinity analysis (Affinity Sensors, Cambridge, UK) indicated the presence of proteins bound to Apl1, a virulence factor of Xanthomonas campestris pv. citri required for the formation of canker symptom on citrus, in the fraction 25–50% ammonium sulfate of citrus crude extracts. Three proteins of 25, 50 and 110-kD were eluted from an Apl1-affinity column. Western analysis revealed that Apl1 binds specifically to the 25-kD and 110-kD but not to the 50-kD protein. When crude extracts of soybean and of tobacco were applied to the Apl1-affinity column, only faint bands were detected. This result suggests that Apl1 targets exist specifically in citrus plants. The amino acid sequence of the N-terminal of the 25-kD protein was determined, and homology search analysis revealed that this sequence was almost identical to those commonly present in S-adenosyl-l-methionine : trans-caffeoyl-coenzyme A 3-O-methyltransferase (CCoAMT) from several plants. This enzyme is specific to the substrate trans -caffeoyl-CoA and catalyzes the synthesis of trans-feruloyl-CoA for lignin formation.

Keywords: citrus canker, Xanthomonas campestris pv. citri, pthA, lignification, S-adenosyl-l-methionine : trans-caffeoyl-coenzyme A 3-O-methyltransferase (CCoAMT), affinity analysis. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© The Phytopathological Society of Japan 2000

Authors and Affiliations

  • Alfredo Gryciuk ALMEIDA
    • 1
  • Kazutoshi SAYAMA
    • 2
  • Shinji TSUYUMU
    • 2
  1. 1.The United Graduate School of Agricultural Science, Gifu University, 1-1, Yanagido, Gifu 501-1193, JapanJP
  2. 2.Faculty of Agriculture, Shizuoka University, 836, Ohya, Shizuoka 422-8529, JapanJP

Personalised recommendations