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Inflammation Research

, Volume 51, Issue 10, pp 483–489 | Cite as

Vitamin E ointment at high dose levels suppresses contact dermatitis in rats by stabilizing keratinocytes

  • K. Kuriyama
  • T. Shimizu
  • T. Horiguchi
  • M. Watabe
  • Y. Abe

Abstract:

Objective: The pharmacological effect of vitamin E ointment at high dose levels was investigated in rats and mice during the development of contact dermatitis. ¶Materials and methods: Allergic or irritant contact dermatitis was induced in sensitized or unsensitized animals by topical application of chemical agent(s). Cultured keratinocytes were prepared from dorsal skin of rats. ¶Results: The vitamin E ointment at 20–40 % suppressed allergic and irritant contact dermatitis, exerting a comparable effect to that of 0.5 % prednisolone ointment. Microscopic findings revealed that 20 % vitamin E ointment reduced the keratinocyte damage, whereas 0.5 % prednisolone was ineffective. The protective action of vitamin E on keratinocyte damage was also confirmed in a cell culture experiment. Furthermore, 20 % vitamin E ointment blocked down-regulation of skin barrier function induced by contact dermatitis, although 0.5 % prednisolone ointment was inactive. ¶Conclusions: These results indicate that 20 % vitamin E ointment suppresses contact dermatitis by stabilizing keratinocytes, concomitantly with novel, interesting properties.

Key words: Vitamin E ointment – High dose levels – Contact dermatitis – Stabilizing action – Keratinocytes 

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Copyright information

© Birkhäuser Verlag, 2002

Authors and Affiliations

  • K. Kuriyama
    • 1
  • T. Shimizu
    • 1
  • T. Horiguchi
    • 1
  • M. Watabe
    • 1
  • Y. Abe
    • 1
  1. 1.Medical Research Laboratory, Sekisui Chemical Co. LTD, 2-1 Hyakuyama Shimamoto-cho, Mishima-gun, Osaka 618, Japan, Fax: ++8175 962 8924, e-mail: kuriyama003@smile2.sekisui.co.jpJP

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