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Clinical and Experimental Nephrology

, Volume 4, Issue 2, pp 119–125 | Cite as

Interleukin 6 as a marker of mesangial cell proliferative activity

  • H. Osawa
  • H. Yamabe
  • M. Kaizuka
  • N. Tamura
  • S. Tsunoda
  • K. Shirato
  • F. Tateyama
  • K. Ozawa
  • K. Okumura
ORIGINAL ARTICLE
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Abstract

Background. The role of Interleukin 6 (IL-6) in the pathogenesis of mesangial proliferative glomerulonephritis has been controversial. To test the hypothesis that glomerular mesangial cell (GMC)-derived IL-6 may not be a mitogen for GMCs, but, rather, a factor that reflects GMC proliferative activity, we investigated how the culture condition; namely, cell confluency, affected IL-6 production in human cultured GMCs.

Methods. IL-6 in the culture supernatant was measured by enzyme-linked immunoassay (ELISA). The expression of IL-6, IL-6 receptor (IL-6R), and proliferating cell nuclear antigen (PCNA) mRNA in GMCs was examined by reverse transcriptase polymerase chain reaction (RT-PCR). The effects of human recombinant IL-6 (rIL-6) on the proliferation of GMCs and an IL-6 dependent cell line, B9 cells, were evaluated by a colorimetric assay.

Results. The IL-6 ELISA study revealed that subconfluent GMCs secreted more IL-6 than confluent GMCs (12.9 ± 2.9 pg/104 cells versus 4.05 ± 0.2 pg/104 cells; P < 0.01). In the RT-PCR, the mRNA expression of both IL-6 and PCNA was upregulated in subconfluent GMCs as compared with confluent GMCs. An approximately four-fold increase in IL-6 mRNA expression and a two-fold increase in PCNA mRNA expression were shown. IL-6R mRNA expression was demonstrated in GMCs by RT-PCR. However, recombinant human IL-6, which stimulated the growth of B9 cells in a dose-dependent fashion, did not induce GMC proliferation.

Conclusions. GMC-derived IL-6 may have significance as a marker of GMC proliferative activity, although GMC-derived IL-6 per se does not act as an autocrine growth factor for GMCs.

Key words Interleukin 6 Interleukin 6 receptor mesangial cell Proliferating cell nuclear antigen 

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Copyright information

© Japan Society of Nephrology 2000

Authors and Affiliations

  • H. Osawa
    • 1
  • H. Yamabe
    • 1
  • M. Kaizuka
    • 1
  • N. Tamura
    • 1
  • S. Tsunoda
    • 1
  • K. Shirato
    • 1
  • F. Tateyama
    • 1
  • K. Ozawa
    • 2
  • K. Okumura
    • 1
  1. 1.Second Department of Internal Medicine, Hirosaki University School of Medicine, 5 Zaifucho, Hirosaki 036-8562, Japan Tel. +81-172-33-5111; Fax +81-172-35-9190 e-mail: osawa@cc.hirosaki-u.ac.jpJP
  2. 2.First Department of Internal Medicine, National Hirosaki Hospital, Hirosaki, JapanJP

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