Advertisement

Marine Biotechnology

, Volume 1, Issue 3, pp 311–315 | Cite as

Isolation and Characterization of Twelve Microsatellite Loci for Rockfish (Sebastes)

  • Peter  Wimberger
  • Jenni  Burr
  • Andy  Gray
  • Andres  Lopez
  • Paul  Bentzen

Abstract:

We describe the first microsatellites for rockfishes in the diverse genus Sebastes. Clones containing microsatellites were isolated from the genomic library of a quillback rockfish, Sebastes maliger. Twelve microsatellites are characterized; six of these are polymorphic in quillback rockfish, and eight are polymorphic in at least one rockfish species on which they were tested. The number of alleles per variable locus ranged from 4 to 15 and averaged 6.8. The expected heterozygosities ranged from 0.38 to 0.79 and averaged 0.60 in these loci. These loci should prove valuable in studies examining species identification, population genetics, hybridization, paternity, kinship, and microsatellite evolution.

Key words:Sebastes, rockfish, microsatellites. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Peter  Wimberger
    • 1
  • Jenni  Burr
    • 1
  • Andy  Gray
    • 2
  • Andres  Lopez
    • 3
  • Paul  Bentzen
    • 3
  1. 1.Department of Biology, University of Puget Sound, Tacoma, WA 98416-0088, U.S.A.US
  2. 2.Fisheries Division, School of Fisheries and Ocean Sciences, University of Alaska Fairbanks, 11120 Glacier Hwy., Juneau, AK 99801, U.S.A.US
  3. 3.Marine Molecular Biology Laboratory, School of Fisheries, University of Washington, 3707 Brooklyn Ave. NE, Seattle, WA 98105-6715, U.S.A.US

Personalised recommendations