Abstract.
A convenient assay method for estimation of barnacle, Balanus amphitrite, settlement inductive activity was developed. To avoid the inductive effect by comrades and laborious observation requirements, cyprid larvae were put individually into wells of a 96-well plate. The settlement ratio from the experiment without any inducers was quite low; therefore this assay allowed easy estimation of settlement inductive activities. Some known inductive agents, such as serotonin and barnacle extracts, clearly showed inductive activities. This assay method is proven to be suitable for estimation of barnacle settlement-inducing activities of both water-soluble and -insoluble compounds.
Author information
Authors and Affiliations
Additional information
Received October 31, 1997; accepted July 10, 1998.
Rights and permissions
About this article
Cite this article
Kawahara, H., Tamura, R., Ajioka, S. et al. Convenient Assay for Settlement Inducing Substances of Barnacles. Mar. Biotechnol. 1, 98–101 (1999). https://doi.org/10.1007/PL00011757
Published:
Issue Date:
DOI: https://doi.org/10.1007/PL00011757