Abstract
A marine Pseudomonas species WAK-1 strain simultaneously produces extracellular glycosaminoglycan and sulfated polysaccharide. Among the antiviral activities tested for these polysaccharides, the latter showed anti-HSV-1 activity in RPMI 8226 cells (50% effective concentration is 1.4 μg/ml). Oversulfated derivatives of these polysaccharides prepared by dicyclohexylcarbodiimide-mediated reaction for both polysaccharides showed antiviral activities against influenza virus type A (for glycosaminoglycan, 50% effective concentration is 11.0 μg/ml; for another, 2.9 μg/ml). Glycosaminoglycan, sulfated polysaccharide, and their chemically synthesized oversulfated derivatives did not show antiviral activities against influenza virus type B and human immunodeficiency virus type 1. No cytotoxicity of these products was noted against host cells at the 50% cytotoxic concentration of 100 μg/ml, except that naturally occurring sulfated polysaccharide had 50% cytotoxicity against MT-4 cells at 8–21 μg/ml.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received May 1, 1998; accepted July 24, 1998.
Rights and permissions
About this article
Cite this article
Matsuda, M., Shigeta, S. & Okutani, K. Antiviral Activities of Marine Pseudomonas Polysaccharides and Their Oversulfated Derivatives. Mar. Biotechnol. 1, 68–73 (1999). https://doi.org/10.1007/PL00011753
Published:
Issue Date:
DOI: https://doi.org/10.1007/PL00011753