The Effect of He-Ne Laser (632.8 nm) and Solcoseryl™ In vitro

Abstract.

He-Ne laser (632.8 nm) and Solcoseryl™ (SS), a non-protein calf haemodialysate, were used in the enhancement of wound healing. Nonetheless, a study on the use of He-Ne laser with SS has not been done. The purpose of this study is to determine the effect of He-Ne laser biostimulation in combination with SS on Chinese hamster ovary (CHO) and human skin fibroblast (HSF). A dose response for the cloning efficiency (CE) of CHO and HSF cells in 5% fetal bovine serum in minimum essential medium (FBS-MEM) with 6–125 µg/ml SS and He-Ne laser using an optimum power density of 1.25 mW/cm² and cumulative doses (CD) of 60–600 mJ/cm² given for three consecutive days, were done. The combined effects of He-Ne laser 180 mJ/cm² with 6 and 12 µg/ml SS were determined. Quadruplicate cultures were done. Student t-test was used to determine differences of treatment groups from controls. CHO and HSF CE were increased using 180 mJ/cm² laser by 13.1%±4.5% (p<0.0025) and 39.1%±7.9% (p<0.0005); SS 6 µg/ml by 14.4%±8.7% (p=0.01) and 20.7%±10.9% (p=0.01); SS 12 µg/ml by 17.7%±6.3% (p=0.001) and 23.9%±5.6% (p<0.0025); laser+SS 6 µg/ml by 15.1%±8.8% (p<0.01) and 60.9%±9.4% (p<0.0001); laser+SS 12 µg/ml by 23.0%±1.5% (p<0.0001) and 70.7%±11.4% (p<0.0001), respectively. Additional significant increases in CE were observed on CHO using laser+SS 12 µg/ml by 8·6%±1·3% (p<0·025) and on HSF using laser+SS 6 µg/ml and laser+SS 12 µg/ml by 15.6%±6.8% (p<0.025) and 22.7%±10.6% (p=0.01), respectively, when compared to the effect of 180 mJ/cm² laser. Results suggest that further stimulation can be achieved by using He-Ne laser with SS. This could be exploited as a new treatment modality.