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Bioprocess Engineering

, Volume 22, Issue 1, pp 35–39 | Cite as

Statistical optimization of lipase catalyzed hydrolysis of methyloleate by response surface methodology

  • M. S. R. C. Murthy
  • T. Swaminathan
  • S. K. Rakshit
  • Y. Kosugi
Article

Abstract

Hydrolysis of methyloleate was optimized using lipase from Chromobacterium viscosum immobilized on IRC-50. The optimization was studied by a statistical methodology using response surface methodology (RSM). The cumulative interactive effect of substrate, water concentration and time was studied in optimizing the hydrolysis of methyloleate. The interactive effect of substrate-time was found to be significant compared to substrate-water and time-water interactions. A well correlation was observed between the optimum values obtained from the response surface contour plots and from the quadratic regression model equation. The optimal values obtained for substrate, water and time were found to be in the experimental range chosen.

Keywords

Hydrolysis Lipase Statistical Optimization Regression Model Interactive Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. S. R. C. Murthy
    • 1
  • T. Swaminathan
    • 1
  • S. K. Rakshit
    • 2
  • Y. Kosugi
    • 3
  1. 1.Biotechnology Research Centre, Department of Chemical Engineering, Indian Institute of Technology, Madras Chennai 600 036, IndiaIN
  2. 2.Bioprocess Technology Programme, Asia Institute of Technology, Bangkok, ThailandTH
  3. 3.NIBH-AIST, l-1 Higashi, Tsukuba, Ibaraki 305, JapanJP

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