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Bioprocess Engineering

, Volume 21, Issue 6, pp 477–481 | Cite as

Optimization of medium components for phytase production by E. coli using response surface methodology

  • K. Sunitha
  • Jung-Kee Lee
  • Tae-Kwang Oh
Article

Abstract

The medium for recombinant phytase production by E. coli BL21 was optimized using response surface methodology. A 23 central composite experimental design was used to study the combined effect of the medium components, tryptone, yeast extract and NaCl. Addition of 2 g/l glucose to the medium greatly influenced the phytase production. The optimization of the medium has increased the phytase production by 1.2 times. The incorporation of 2 g/l glucose significantly enhanced the phytase production by 1.58 times, showing an overall 2.78 fold increase before optimization and other modifications of the medium. The optimized medium with glucose showed a highest phytase activity of 2250 U/l.

Keywords

Glucose Combine Effect Yeast Extract Response Surface Fold Increase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • K. Sunitha
    • 1
  • Jung-Kee Lee
    • 1
  • Tae-Kwang Oh
    • 1
  1. 1.Microbial Enzyme RU, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea e-mail: otk@kribb4680.kribb.re.krKR

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