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Bioprocess Engineering

, Volume 21, Issue 5, pp 415–421 | Cite as

Box-Behnken design in the development of optimized complex medium for phenol degradation using Pseudomonas putida (NICM 2174)

  • G. Annadurai
  • S. Mathalai Balan
  • T. Murugesan
Article

Abstract

The biodegradation of phenol by Pseudomonas putida (NICM 2174), a potential biodegradent of phenol has been investigated for its degrading potential under different operating conditions. Box-Behnken design has been employed to study the effect of different experimental variables. Four variables of maltose (0.25, 0.5, 0.75 g/l), phosphate (3, 12.5, 22 g/l), pH (7, 8, 9) and temperature (30 °C, 32 °C, 34 °C) were used to identify the significant effects and interactions in the batch studies. A second order polynomial regression model, has been developed using the experimental data. It was found that the degrading potential of Pseudomonas putida (NICM 2174) was strongly affected by the variations in maltose, phosphate, pH and temperature. The experimental values were in good agreement with the predicted values, the correlation coefficient was found to be 0.9980. Optimum conditions of the variables for the growth of Pseudomonas putida (NICM2174) and for maximum biodegradation of phenol are maltose (0.052 g/l), phosphate (8.97 g/l), pH (7.9) and temperature (31.5 °C).

Keywords

Phosphate Experimental Data Phenol Optimum Condition Regression Model 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • G. Annadurai
    • 1
  • S. Mathalai Balan
    • 1
  • T. Murugesan
    • 1
  1. 1.Department of Chemical Engineering, Alagappa College of Engineering, Anna University, Chennai - 600 025, IndiaIN

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