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Bioprocess Engineering

, Volume 21, Issue 3, pp 227–230 | Cite as

Effect of oxygen enrichment aeration on penicillin G acylase production in high cell density culture of recombinant E. coli

  • Y.-C. Liu
  • W.-M. Chang
  • C.-Y. Lee
Article

Abstract

A strain of E. coli 9633 harboring pGL-5 is employed to develop the process producing penicillin G acylase (PGA). Medium studies show that 0.8% glucose is a proper initial carbon source concentration in the start medium. Whereas, study of nitrogen sources in the production medium exhibits that 2% soybean meat hydrolysate and 0.5% casein hydrolysate give the best result in terms of cell concentration. High cell density culture is carried out through the application of oxygen enrichment aeration (OEA). With the treatment of OEA, the cell concentration and specific PGA activity raise to 2.1 and 1.4 times, respectively, as compared to those without OEA. In a 75 l fermentor, the cell concentration can reach 323 (g wet cell weight/l) with a specific PGA activity of 47 (IU/g w.w.) after 24 h under OEA treatment. This means that the PGA activity of the broth can reach 15 (IU/ml).

Keywords

Nitrogen Source Cell Concentration Medium Study Production Medium Casein Hydrolysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Y.-C. Liu
    • 1
  • W.-M. Chang
    • 2
  • C.-Y. Lee
    • 2
  1. 1.Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, ROCTW
  2. 2.Union Chemical Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan, ROCTW

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