Optimal mode of operation for temperature induction of bacteriophage λQ− mutant in Escherichia coli
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An epidemic model is presented to provide theoretical considerations for temperature induction of bacteriophage λ in Escherichia coli. Based upon model predictions for bioreactor operations, the optimal mode for reporter-protein induction is fed-batch since augmentation of susceptible cells enhances λ infection in batch cultures and washout of infected cells decreases induction performance in CSTR operations. Experimental results also support that batch mode of operation is better than CSTR.
KeywordsEscherichia Coli Model Prediction Infected Cell Washout Batch Culture
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