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Bioprocess Engineering

, Volume 17, Issue 4, pp 197–202 | Cite as

The effect of oxygen transfer on the activity of encapsulated whole cell β-galactosidase

  • J. K. Park
  • G. S. Jeong
  • H. N. Chang

Abstract

The effect of oxygen transfer on the production of immobilized whole cell β-galactosidase has been evaluated. The encapsulated whole cell β-galactosidase was prepared by combining cell encapsulation and culture into one-step. Escherichia coli was encapsulated and cultured in the growth and production media to accumulate β-galactosidase in itself. Sunflower seed oil was coimmobilized to increase the oxygen transfer rate through the capsule membrane. The oxygen transfer rate increased 63 percent and the activity of β-galactosidase increased by 10 percent. The activity of encapsulated β-galactosidase obtained in the concentric air lift reactor was 86 percent higher than that in the shaking incubator. In the concentric air lift reactor, the accumulation of encapsulated whole cell β-galactosidase was primarily dependent on the capsule velocity. While the accumulation of specific β-galactosidase in the capsule increased with volumetric oxygen transfer coefficient, the cell biomass accumulated in the capsule decreased.

Keywords

Biomass Escherichia Coli Transfer Coefficient Encapsulation Production Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • J. K. Park
    • 1
  • G. S. Jeong
    • 1
  • H. N. Chang
    • 2
  1. 1.Dept. of Chem. Eng., Kyungpook National University, 702-701 Taegu, KoreaKR
  2. 2.BioProcess Engineering Research Center and Dept. of Chemical Engineering, KAIST, Daedok Science Town, 305-701 Taejon, KoreaKR

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