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Molecular and General Genetics MGG

, Volume 263, Issue 6, pp 995–1002 | Cite as

Both sense and antisense RNAs are targets for the sense transgene-induced posttranscriptional silencing mechanism

  • H. Van Houdt
  • M. Van Montagu
  • A. Depicker
ORIGINAL PAPER

Abstract

Two stable transgenic tobacco lines were obtained as segregants from a primary transformant. Plants homozygous for a T-DNA inverted repeat locus (HOlo1) showed posttranscriptional gene silencing (PTGS) of the neomycin phosphotransferase II (nptII) transgenes, whereas HOlo2 plants, homozygous for a single T-DNA insert, expressed the nptII genes normally. Transient expression of nptII genes newly introduced into leaves of both the HOlo2 and nptII-silenced HOlo1 plants was downregulated only in the silenced background. Different chimeric β-glucuronidase (gus) genes with parts of the nptII transgene inserted in sense or antisense orientation into the 3′-untranslated region, which encoded transcripts that had homology or complementarity to nptII transcripts, showed reduced transient expression specifically in nptII-silenced tissue. Therefore, we conclude that RNAs of both polarities are targets for PTGS-induced RNA degradation, which supports the notion that double-stranded RNA acts as an inducing signal for silencing.

Key words Complementary RNA Cosuppression RNA turnover Transgenic plant Transient expression 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • H. Van Houdt
    • 1
  • M. Van Montagu
    • 1
  • A. Depicker
    • 1
  1. 1.Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium E-mail: anna.depicker@rug.ac.be Tel.: +32-9-2645174; Fax: +32-9-2645349BE

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