Advertisement

Molecular and General Genetics MGG

, Volume 256, Issue 2, pp 179–186 | Cite as

Molecular characterization of the glnA gene encoding glutamine synthetase from the edible mushroom Agaricus bisporus

  • M. A. S. H. Kersten
  • Y. Müller
  • H. J. M. Op den Camp
  • G. D. Vogels
  • L. J. L. D. Van Griensven
  • J. Visser
  • P. J. Schaap
ORIGINAL PAPER

Abstract

The gene encoding glutamine synthetase (glnA) was isolated from an Agaricus bisporus H39 recombinant λ phage library. The deduced A. bisporus glutamine synthetase amino acid sequence contains 354 residues. The amino acid sequence is very similar to that derived from the gene coding for glutamine synthetase of the yeast Saccharomyces cerevisiae. The open reading frame is interrupted by four introns. Northern analysis revealed that transcription of the gene is repressed upon addition of ammonium to the culture but the repression was not as strong as that of the gene encoding NADP+-dependent glutamate dehydrogenase (gdhA). Enzyme activities are low in the presence of ammonium, glutamine and albumin and do not correlate with the mRNA levels revealed by Northern analysis. This suggests that glutamine synthetase expression in A. bisporus is also post-transcriptionally regulated by the nitrogen source.

Key wordsAgaricus bisporus Glutamine synthetase Molecular cloning Gene structure Mushroom 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • M. A. S. H. Kersten
    • 1
  • Y. Müller
    • 2
  • H. J. M. Op den Camp
    • 1
  • G. D. Vogels
    • 1
  • L. J. L. D. Van Griensven
    • 3
  • J. Visser
    • 2
  • P. J. Schaap
    • 2
  1. 1.Department of Microbiology and Evolutionary Biology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The NetherlandsNL
  2. 2.Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, Dreijenlaan 2, NL-6703 HA Wageningen, The Netherlands Fax +31 (0) 317 484011NL
  3. 3.Mushroom Experimental Station, P.O. Box 6042, NL-5960 AA Horst, The NetherlandsNL

Personalised recommendations