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Eligibility of antigenic-peptide-pre-loaded and fixed adhesive peripheral blood cells for induction of cytotoxic T lymphocytes from cancer patients with elevated serum levels of carcinoembryonic antigen

  • Chang Hyun Kim
  • Takeshi Todoroki
  • Masatoshi Matsumura
  • Tadao Ohno
ORIGINAL PAPER

Abstract

The inducibility of cytotoxic T lymphocytes (CTL) that react with carcinoembryonic antigen (CEA) was tested in cancer patients with elevated (more than 5 ng/ml) serum CEA levels when antigen presentation was carried out with paraformaldehyde-fixed adhesive peripheral blood mononuclear cells (PBMC) from the patient that had been pre-loaded with CEA652(9), an HLA-A2402-restricted tumor antigenic peptide derived from CEA. By culturing fresh autologous PBMC on the fixed cell layer in medium containing interleukin-1, -2, -4 and -6, three out of eight patients developed CTL. The CTL from two of these patients killed CEA-protein-producing gastric cancer cells carrying HLA-A2402 and the cells from the remaining patient killed CEA-non-producing stomach cancer cells pre-loaded with CEA652(9). The results suggest that a single antigenic peptide on the fixed adhesive cells will allow the ex vivo induction of peptide-reactive CTL that are easier to handle and allow antigen presentation without tedious preculture of the “professional” antigen-presenting dendritic-cells.

Key words Carcinoembryonic antigen Cytotoxic T lymphocyte HLA-A2402 Epitope peptide Immunotherapy 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Chang Hyun Kim
    • 1
  • Takeshi Todoroki
    • 2
  • Masatoshi Matsumura
    • 3
  • Tadao Ohno
    • 1
  1. 1.RIKEN Cell Bank, RIKEN (The Institute of Physical and Chemical Research), Koyadai, Tsukuba Science City, 305-0074, Japan e-mail: tad-ohno@rtc.riken.go.jp Tel.: +81-298-36-9124 Fax: +81-298-36-9049JP
  2. 2.Department of Surgery, Institute of Clinical Medicine, University of Tsukuba, Tennodai, Tsukuba Science City, 305-0074, JapanJP
  3. 3.Institute of Applied Biochemistry University of Tsukuba, Tennodai, Tsukuba Science City, 305-0074, JapanJP

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