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Archives of Gynecology and Obstetrics

, Volume 266, Issue 1, pp 34–37 | Cite as

Circulating CD34+ cells to predict the adequate harvest of peripheral blood progenitor cells in platinum-based chemotherapy

  • H. Kurata
  • K. Takakuwa
  • I. Tsuneki
  • Y. Aoki
  • K. Tanaka
Original Article

Abstract  

Objective: The aim of this study was to clarify the appropriate timing for peripheral blood progenitor cells (PBPC) harvesting after platinum-based mobilization chemotherapy by measurement of the circulating CD34+ cell concentrations. Patients and Methods: PBPC were collected for autotransplantation via a total of 68 leukaphereses in 16 patients with gynecological cancer. Circulating CD34+ cell concentrations were measured by CD34-side scatter parameter analysis. Results: Data could be fitted into a linear regression line described by the equation y=0.33+4.14×x (R2=0.256), where y=the number of harvested colony-forming unit granulocyte macrophage (CFU-GM (×105/kg) and x=the percentage of circulating CD34+ cells and y=1.747+44.53×x (R2= 0.475), where y=the number of harvested CD34+ cells (×106/kg) and x=the percentage of circulating CD34+ cells. Failure to mobilize sufficient CFU-GM numbers (>1×105/kg) occurred in 29 of 31 leukaphereses when the percentage of circulating CD34+ cells was less than 0.10%. Conclusions: Harvesting procedure may be avoided when circulating CD34+ cells showed less than 0.10% after platinum-based mobilization chemotherapy.

Keywords PBPC harvest CD34 CFU-GM Platinum-based chemotherapy 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • H. Kurata
    • 1
  • K. Takakuwa
    • 1
  • I. Tsuneki
    • 1
  • Y. Aoki
    • 1
  • K. Tanaka
    • 1
  1. 1.Department of Obstetrics and Gynecology, Niigata University Faculty of Medicine, Asahimachi-dori 1-757, Niigata, 951-8510, Japan e-mail: tounin@med.niigata-u.ac.jp Tel.: 25-227-2320, Fax: 25-227-0789JP

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