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Acta Neuropathologica

, Volume 99, Issue 3, pp 305–309 | Cite as

Glial pathology in development of cerebellar dysplasia in the hereditary cerebellar vermis defect (CVD) rat

  • M. Kuwamura
  • T. Morikawa
  • J. Yamate
  • K. Kato
  • T. Kotani
  • S. Sakuma
Regular paper

Abstract

The cerebellar vermis defect (CVD) rat is a new neurological mutant characterized by a cerebellar vermis defect and dysplasia in the cerebellum, especially at the cerebellopontine junctions. In this study, the cytokinetics of glia in terms of the development of cerebellar dysplasia in the CVD rat was investigated using glial fibrillary acidic protein (GFAP) and vimentin immunohistochemistry. In the cerebellar hemispheres, dislocation of the Bergmann glia was observed from postnatal day 5 (P5) in lesions with abnormally aggregated external granule cells (EGCs). Rearranging Bergmann glia were often seen around the EGCs penetrating into the white matter. In the cerebellopontine junctional areas, Bergmann glia were induced after penetration of the Purkinje cells, identified with calbindin immunohistochemistry, and EGCs into the pons from P10. Bergmann fibers were frequently arranged perivascularly. In the clusters of Purkinje cells without EGC settlement in the pons, a small number of Bergmann fibers were observed and their alignment was completely disturbed. These findings suggest that morphological changes in the Bergmann glia depend on their contact with Purkinje cells, but that the orientation of their processes may be influenced by EGC settlement. These glial fibers in the CVD rat may play an important role in the aberrant migration of EGCs, resulting in the development of cerebellar dysplasia.

Key words Bergmann glia Cell migration Cerebellar ¶dysplasia Immunohistochemistry Mutant rat 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. Kuwamura
    • 1
  • T. Morikawa
    • 1
  • J. Yamate
    • 1
  • K. Kato
    • 2
  • T. Kotani
    • 1
  • S. Sakuma
    • 1
  1. 1.Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan e-mail: kuwamura@vet.osakafu-u.ac.jp, Tel.: +81-722-549482, Fax: +81-722-507208JP
  2. 2.Department of General Science, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, JapanJP

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