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Current Microbiology

, Volume 38, Issue 4, pp 217–223 | Cite as

Fingerprinting of Mixed Bacterial Strains and BIOLOG Gram-Negative (GN) Substrate Communities by Enterobacterial Repetitive Intergenic Consensus Sequence-PCR (ERIC-PCR)

  • George D.  Di Giovanni
  • Lidia S.  Watrud
  • Ramon J.  Seidler
  • Franco  Widmer

Abstract.

PCR-based genomic fingerprinting by use of enterobacterial repetitive intergenic consensus primers (ERIC-PCR) was evaluated for its use in fingerprinting DNA of mixed Gram-negative bacterial strains and BIOLOG Gram-negative (GN) microplate substrate communities. ERIC-PCR fingerprints of six different pure bacterial strains and a combined mixture of the strains were compared with fingerprints obtained by two more established methods: amplified ribosomal DNA restriction analysis (ARDRA) and random amplified polymorphic DNA analysis (RAPD-PCR). The ERIC-PCR fingerprint of the mixed strains was highly reproducible and was more species-specific and representative of the individual strain fingerprints than the ARDRA and RAPD-PCR fingerprints, respectively. ERIC-PCR fingerprinting of model and rhizosphere BIOLOG GN substrate communities also provided clearly distinguishable fingerprints. Results of this study suggest that ERIC-PCR represents a rapid and highly discriminating method for fingerprinting DNA of mixed Gram-negative bacterial strains and BIOLOG GN substrate communities.

Keywords

Individual Strain Consensus Primer Genomic Fingerprinting Enterobacterial Repetitive Intergenic Consensus Mixed Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • George D.  Di Giovanni
    • 1
  • Lidia S.  Watrud
    • 2
  • Ramon J.  Seidler
    • 2
  • Franco  Widmer
    • 1
  1. 1.National Research Council Research Associate, US EPA National Health and Environmental Effects Research Laboratory–Western Ecology Division, 200 SW 35th Street, Corvallis, OR, USA US
  2. 2.US EPA National Health and Environmental Effects Research Laboratory–Western Ecology Division, 200 SW 35th Street, Corvallis, OR, USA US

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