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Current Microbiology

, Volume 38, Issue 1, pp 18–21 | Cite as

Purification and Characterization of a Low-Molecular-Weight Xylanase Produced by Acrophialophora nainiana

  • Fabiano de Aquino  Ximenes
  • Marcelo Valle  de Sousa
  • Jürgen  Puls
  • Francides Gomes  da Silva Jr.
  • Edivaldo Ximenes Ferreira  Filho

Abstract.

A low-molecular-weight xylanase activity (XynI) was isolated from the fungus Acrophialophora nainiana after growth in a solid medium containing wheat bran. XynI was purified to apparent homogeneity by ultrafiltration and gel filtration chromatography. The purified enzyme had a molecular weight value of approx. 17 kDa, as determined by SDS-PAGE. This enzyme was most active at 50°C and pH 6.0. At 50°C the half-life was 150 min. The apparent K m value for birchwood xylan was much lower than the K m value for oat spelt xylan. XynI was activated by L-cysteine, DTE, β-mercaptoethanol, and L-tryptophan. XynI did not show significant sequence homology with other xylanases. The analysis of hydrolysis products of xylans and wood pulps showed that XynI was able to release xylooligomers ranging from X2 to X3 and X2 to X6, respectively. The enzyme was not active against acetylated xylan. A small amount of xylose was released from deacetylated, birchwood, and oat spelt xylans. The results obtained with enzymatic treatment of Kraft pulp indicated a reduction in the amount of chlorine compounds required for the process and enhanced brightness gain.

Keywords

Enzyme Xylose Bran Hydrolysis Product Wheat Bran 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Fabiano de Aquino  Ximenes
    • 1
  • Marcelo Valle  de Sousa
    • 2
  • Jürgen  Puls
    • 3
  • Francides Gomes  da Silva Jr.
    • 4
  • Edivaldo Ximenes Ferreira  Filho
    • 1
  1. 1.Laboratório de Enzimologia, Departamento de Biologia Celular, Universidade de Brasília, DF, Brasil BR
  2. 2.Centro Brasileiro de Serviços e Pesquisas em Proteínas, Departamento de Biologia Celular, Universidade de Brasília, DF, Brasil BR
  3. 3.Institut für Holzchemie, D-2050 Hamburg 80, Germany DE
  4. 4.Laboratório de Desenvolvimento de Processos/Produtos-Celulose, Votorantim Celulose & Papel, Luis Antonio–SP, Brasil BR

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