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Journal of Molecular Evolution

, Volume 44, Issue 1, pp 81–88 | Cite as

Contribution of Mutation and RNA Recombination to the Evolution of a Plant Pathogenic RNA

  • Miguel A.  Aranda
  • Aurora  Fraile
  • Joaquín  Dopazo
  • José M.  Malpica
  • Fernando  García-Arenal
Article

Abstract.

The nucleotide sequence of 17 variants of the satellite RNA of cucumber mosaic virus (CMV-satRNA) isolated from field-infected tomato plants in the springs of 1989, 1990, and 1991 was determined. The sequence of each of the 17 satRNAs was unique and was between 334 and 340 nucleotides in length; 57 positions were polymorphic. There was much genetic divergence, ranging from 0.006 to 0.141 nucleotide substitutions per site for pairwise comparisons, and averaging 0.074 for any pair. When the polymorphic positions were analyzed relative to a secondary structure model proposed for CMV-satRNAs, it was found that there were significantly different numbers of changes in base-paired and non–base-paired positions, and that mutations that did not disrupt base pairing were preferred at the putatively paired sites. This supports the concept that the need to maintain a functional structure may limit genetic divergence of CMV-satRNA. Phylogenetic analyses showed that the 17 CMV-satRNA variants clustered into two subgroups, I and II, and evolutionary lines proceeding by the sequential accumulation of mutations were apparent. Three satRNA variants were outliers for these two phylogenetic groups. They were shown to be recombinants of subgroup I and II satRNAs by calculating phylogenies for different molecular regions and by using Sawyer's test for gene conversion. At least two recombination events were required to produce these three recombinant satRNAs. Thus, recombinants were found to be frequent (∼17%) in natural populations of CMV-satRNA, and recombination may make an important contribution to the generation of new variants. To our knowledge this is the first report of data allowing the frequency of recombinant isolates in natural populations of an RNA replicon to be estimated.

Key words: Nucleotide sequence — Cucumber mosaic virus — Plant pathogenic RNA 

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Copyright information

© Springer-Verlag New York Inc. 1997

Authors and Affiliations

  • Miguel A.  Aranda
    • 1
  • Aurora  Fraile
    • 1
  • Joaquín  Dopazo
    • 2
  • José M.  Malpica
    • 3
  • Fernando  García-Arenal
    • 1
  1. 1.Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, 28040 Madrid, SpainES
  2. 2.Unidad de Bioinformática, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, SpainES
  3. 3.Area de Biología Molecular y Virología Vegetal, Centro de Investigación y Tecnología-Instituto Nacional de Investigacines Agrarias, 28040, Madrid, SpainES

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