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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 359, Issue 3, pp 178–186 | Cite as

Effects of antidepressant drugs on the dopamine D2/D3 receptors in the rat brain differentiated by agonist and antagonist binding – an autoradiographic analysis

  • Renata Rogoz
  • M. Dziedzicka-Wasylewska
Original article

Abstract

In the present study the effect of antidepressant drugs on the density of dopamine D2/D3 receptors in the rat forebrain was examined using autoradiography, since this technique provides the appropriate anatomical resolution. Male Wistar rats were treated with three various antidepressant drugs: imipramine, citalopram and mianserin in a dose of 10 mg/kg p.o., acutely (single dose) or repeatedly (twice a day for 14 days). To estimate the distribution of D2/D3 receptors, we chose following radioligands: [3H]raclopride, a non-selective antagonist of D2/D3 receptors, and [3H]quinpirole, a non-selective D2/D3 agonist. When [3H]raclopride was used as a radioligand, no significant differences in the density of D2/D3 receptors were observed after administration of the investigated drugs. However, following repeated administration of imipramine, citalopram and mianserin, a significant increase in the binding of [3H]quinpirole was observed, both in the nucleus caudatus and nucleus accumbens septi. In some cases the increase of [3H]quinpirole binding was also observed after acute treatment with antidepressant drugs. Thus, using an agonist as a radioligand, we were able to see upregulation of dopamine D2/D3 receptors in the rat forebrain following administration of antidepressant drugs, which might be interpreted as the biochemical correlative for the postsynaptic dopamine D2/D3 receptor supersensitivity observed in previous behavioral studies.

Key words Antidepressants Dopamine receptors Autoradiography Rat brain 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Renata Rogoz
    • 1
  • M. Dziedzicka-Wasylewska
    • 1
  1. 1.Department of Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, 12 Sm´tna Street, PL-31-343 Kraków, Poland Fax: +48-12-6374500PL

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