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Influence of blockade of α1-adrenoceptors, β1-adrenoceptors and vasopressin V1A receptors on the cardiovascular effects of γ2-melanocyte-stimulating hormone (γ2-MSH)

  • Patricia Van Bergen
  • Tessa Y. C. E. De Winter
  • D. J. De Wildt
  • D. H. G. Versteeg
  • D. J. De Wildt
Original article

Abstract

γ2-Melanocyte-stimulating hormone (γ2-MSH) and related melanotropins have been shown to have various cardiovascular effects, including acute, short-lasting increases in blood pressure (MAP) and heart rate (HR). γ2-MSH, administered intravenously, dose-dependently increased MAP and HR with an ED50 of approximately 30 nmol/kg and a maximal effect on MAP of approximately 55 mm Hg and on HR of around 70 beats per minute. Intravenous (i.v.) pretreatment with the α1-adrenoceptor antagonist, prazosin, caused the dose-response curve for the effect of γ2-MSH on MAP to shift to the right with a decrease in slope, whereas it had no effect on the dose-response curve for the effect on HR. I.v. pretreatment with the β1-adrenoceptor antagonist, metoprolol, had no effect on the dose-response curve for the effect of γ2-MSH on MAP, but it caused the dose-response curve for the effect of the peptide on HR to shift to the right with a decrease in slope. Neither i.v. nor intracerebroventricular (i.c.v.) administration of the vasopressin V1A receptor antagonist, SR 49059 ((2S) 1-[(2R 3S)-5-chloro-3-(2-chlorophenyl)-1-(3,4-dimethoxybenzene-sulfonyl)-3-hydroxy-2,3-dihydro-1H-indole-2-carbonyl]-pyrrolidine-2-carboxamide), had significant effects on the dose-response curves for the effects of the peptide on either MAP or HR. The doses of prazosin, metoprolol and SR 49059 were found to be effective in counteracting the effects of agonists for these receptors (phenylephrine, isoprenaline and [Arg8]vasopressin, respectively). Taken together, these results support the postulate that the effects of γ2-MSH are, at least partially, due to an increase in sympathetic outflow to the periphery (Gruber and Callahan (1989), Am J Physiol 257: R681-R694), and that this increase leads to increased activation of vascular α1-adrenoceptors and cardiac β1-adrenoceptors. If, as was suggested by these authors, γ2-MSH acts via activation of a central vasopressin system, it is via a vasopressin receptor subtype other than the vasopressin V1A receptor, since i.c.v. administration of a selective vasopressin V1A receptor antagonist failed to interfere with the pressor and cardioaccelerator effects of γ2-MSH.

Key words γ2-MSH (γ2-melanocyte-stimulating hormone) Blood pressure Heart rate Prazosin Metoprolol SR 49059 Rat 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Patricia Van Bergen
    • 1
  • Tessa Y. C. E. De Winter
    • 1
  • D. J. De Wildt
    • 1
  • D. H. G. Versteeg
    • 1
  • D. J. De Wildt
    • 2
  1. 1.Department of Medical Pharmacology, Rudolf Magnus Institute for Neurosciences, Utrecht University, Universiteitsweg 100, 3584 CG Utrecht, The NetherlandsNL
  2. 2.Laboratory for Pharmacology and Toxicology, National Institute of Public Health and Environmental Protection, Antonie van Leeuwenhoeklaan 9, 3720 MA Bilthoven, The NetherlandsNL

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