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Theoretical and Applied Genetics

, Volume 103, Issue 4, pp 601–606 | Cite as

Genetic analysis of kernel hardness in bread wheat using PCR-based markers

  • A. A. Galande
  • R. Tiwari
  • J. S. S. Ammiraju
  • D. K. Santra
  • M. D. Lagu
  • V. S. Rao
  • V. S. Gupta
  • B. K. Misra
  • S. Nagarajan
  • P. K. Ranjekar

Abstract 

In wheat, kernel hardness is a complex genetic trait involving various directly and indirectly contributing components such as kernel hardness per se, protein content, hectolitre weight and 1,000-kernel weight. In an attempt to identify DNA markers associated with this trait, 100 recombinant inbred lines (RILs) derived from a cross between a hard grain land-race, NP4, and a soft grain variety, HB 208, were screened with 100 ISSR and 360 RAPD primers. Eighteen markers were assigned to seven linkage groups covering 223.6 cM whereas 11 markers remained unlinked. A multiple-marker model explained the percentage of phenotypic variation for kernel hardness as 20.6%, whereas that for protein content, hectolitre weight and 1,000-kernel weight was 18.8%, 13.5% and 12.1%, respectively. Our results indicate that phenotypic expression of kernel hardness is controlled by many QTLs and is interdependent on various related traits.

Keywords Wheat Kernel hardness (KH) Quantitative trait loci (QTL) RAPD ISSR Recombinant Inbred lines (RILs) 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • A. A. Galande
    • 1
  • R. Tiwari
    • 2
  • J. S. S. Ammiraju
    • 1
  • D. K. Santra
    • 1
  • M. D. Lagu
    • 1
  • V. S. Rao
    • 3
  • V. S. Gupta
    • 1
  • B. K. Misra
    • 2
  • S. Nagarajan
    • 2
  • P. K. Ranjekar
    • 1
  1. 1.Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, India e-mail: pkr@ems.ncl.res.in Fax: +91-20-5884032IN
  2. 2.Directorate of Wheat Research, Karnal 132 001, IndiaIN
  3. 3.Agharkar Research Institute, Pune 411 004, IndiaIN

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