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Cellular and Molecular Life Sciences CMLS

, Volume 58, Issue 14, pp 2098–2107 | Cite as

Role of phospholipid transfer protein in rabbit lung development

  • S. Kaushal
  • S. Ghosh
  • N. Sharma
  • S. N. Sanyal
  • S. Majumdar

Abstract.

A 36-kDa phospholipid transfer protein (PLT-PR), which preferentially transfers phosphatidyl choline (PC) compared to phosphatidyl inositol (PI), was purified 827-fold from rabbit lung homogenate. Incorporation of cholesterol in unilamellar vesicles reduced the PC transfer activity of PLTPR. Dipalmitoyl phosphatidyl choline uptake by alveolar type II cells was increased in the presence of the protein, and further enhanced in the presence of surfactant liposomes. However, a decrease in uptake was noted with cholesterol in host membranes. Incorporation of PI into host membranes had a low stimulatory effect on the process. All these effects were more pronounced in adult type II cells compared to premature, term and 3-day-old pups.

Key words. Phospholipid transfer protein; Dipalmitoyl phosphatidyl choline; surfactant; respiratory distress syndrome. 

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Copyright information

© Birkhäuser Verlag, 2001

Authors and Affiliations

  • S. Kaushal
    • 1
  • S. Ghosh
    • 1
  • N. Sharma
    • 1
  • S. N. Sanyal
    • 2
  • S. Majumdar
    • 1
  1. 1.Department of Experimental Medicine & Biotechnology, PGIMER, Chandigarh 160012 (India), Fax +91 172 747 403, e-mail: medinst@pgi.chd.nic.inIN
  2. 2.Department of Biophysics, Panjab University, Chandigarh (India)IN

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