Advertisement

Cellular and Molecular Life Sciences CMLS

, Volume 58, Issue 1, pp 135–140 | Cite as

T-antigen regulated expression reduces apoptosis of Tag-transformed human myoblasts

  • S. Corti*
  • S. Salani
  • R. Del Bo
  • Y. Torrente
  • S. Strazzer
  • M. Belicchi
  • S. Paganoni
  • Z. Li
  • G. P. Comi
  • N. Bresolin
  • D. Paulin
  • G. Scarlato

Abstract.

The generation of human myogenic cell lines could potentially provide a valuable source for cell transplantation in myopathies. The dysregulation of proliferative-differentiative signals by viral oncogenes can result in the induction of apoptosis. Whether apoptosis occurred in myogenic cells expressing large T antigen (Tag) from SV40 upon differentiation was unknown. Human muscle satellite cells were transfected with two different constructs, containing either an origin-defective SV40 genome or Tag under vimentin promoter control. When differentiation was triggered, Tag expression reduced the formation of myotubes and dead cells showing apoptotic features were present. However, the cells expressing SV40 Tag under vimentin promoter control retained their capacity to form myotubes and expressed the myofibrillar proteins as myosin heavy chain and dystrophin when Tag expression was silent. Their apoptotic rate was similar to that of untransfected cells. The observation that apoptosis can be prevented by the down-regulation of Tag suggests that the programmed cell death induced in transformed cells can be reversed, and confirms the regulatory efficiency of the human vimentin promoter.

Key words. Myogenic cells; SV40 large T antigen; apoptosis; vimentin; myotube. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Birkhäuser Verlag Basel, 2001

Authors and Affiliations

  • S. Corti*
    • 1
  • S. Salani
    • 2
  • R. Del Bo
    • 1
  • Y. Torrente
    • 1
  • S. Strazzer
    • 2
  • M. Belicchi
    • 1
  • S. Paganoni
    • 1
  • Z. Li
    • 3
  • G. P. Comi
    • 1
  • N. Bresolin
    • 1
  • D. Paulin
    • 3
  • G. Scarlato
    • 1
  1. 1.Institute of Clinical Neurology, IRCCS Ospedale Maggiore Policlinico, University of Milan, Via Francesco Sforza 35, 20122 Milan (Italy), Fax +39 02 55190392, e-mail: ticor@tin.it IT
  2. 2.IRCCS Eugenio Medea, Bosisio Parini (Italy) IT
  3. 3.Université Paris 7, 2 place Jussieu, 75005 Paris (France) FR

Personalised recommendations