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Cellular and Molecular Life Sciences CMLS

, Volume 57, Issue 7, pp 1103–1108 | Cite as

Expression and cellular distribution of perchloric acid-soluble protein is dependent on the cell-proliferating states of NRK-52E cells

  • H. Kanouchi
  • T. Oka
  • K. Asagi
  • H. Tachibana
  • K. Yamada*

Abstract.

To clarify the biological role of kidney perchloric acid-soluble protein 1 (K-PSP1), its expression and intracellular distribution were examined in normal rat kidney epithelial NRK-52E cells. K-PSP1 expression was low during the proliferating phase and high in the stationary phase, and shown to have a negative relationship with the protein-synthesizing activity of the cells. Immunocytochemical studies revealed that K-PSP1 is predominantly located in the cytosol, especially in endoplasmic reticulum and Golgi apparatus of proliferating cells. In the stationary phase, K-PSP1 was not detected immunologically even though protein and mRNA expression were high. This disappearance of reactivity with anti-serum seems to be due to a conformational change in K-PSP1 induced by unknown factors. These results suggest that the role of K-PSP1 is to regulate cell proliferation, and this may be related to a previously reported ability to inhibit protein synthesis.

Key words. Perchlolic acid-soluble protein; protein synthesis inhibitor; cell growth; immunoblotting; quantitative RT-PCR reaction; immunofluorescent cytochemistry. 

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Copyright information

© Birkhäuser Verlag Basel, 2000

Authors and Affiliations

  • H. Kanouchi
    • 1
  • T. Oka
    • 2
  • K. Asagi
    • 3
  • H. Tachibana
    • 1
  • K. Yamada*
    • 1
  1. 1.Laboratory of Food Chemistry, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School of Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 (Japan), Fax +81 92 642 3007, e-mail: yamadako@agr.kyushu-u.ac.jpJP
  2. 2.Department of Veterinary Physiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065 (Japan)JP
  3. 3.Otsuka Pharmaceutical Factory, Inc., Nutrition Research Institute, 115 Aza Kuguhara Tateiwa, Muya-cho, Naruto-shi, Tokushima 772-8601 (Japan)JP

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