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Cellular and Molecular Life Sciences CMLS

, Volume 53, Issue 3, pp 271–274 | Cite as

Cucurbitane-type compounds from Hemsleya carnosiflora antagonize ecdysteroid action in the Drosophila melanogaster BII cell line

  • L. Dinan
  • P. Whiting
  • S. D. Sarker
  • R. Kasai
  • K. Yamasaki

Abstract.

The ecdysteroid agonist and antagonist activities of 3 cucurbitanes, 2 cucurbitane glycosides and 2 cucurbitacins isolated from Hemsleya carnosiflora (Cucurbitaceae) have been determined in the Drosophila melanogaster BII bioassay. Carnosiflogenins A and C and carnosiflosides II and VI possess antagonistic activity. Carnosiflogenin A was also found to induce the formation of spindle-shaped cells with high frequency in both the agonist and antagonist assays. At 10−3 M, carnosiflogenins B and C were cytotoxic. 23,24-Dihydrocucurbitacin F and 25-acetoxy-23,24-dihydrocucurbitacin F are also antagonistic at high concentrations. The concentration dependencies of the antagonistic activities of these two cucurbitacins, carnosiflosides II and VI and carnosiflogenin C are presented. The biological and ecological significance of these results are discussed in relationship to the concentrations present in the rhizomes of H. carnosiflora.

Key words. Antagonist; Cucurbitaceae; cucurbitane; cucurbitacin; ecdysteroid; Hemsleya carnosiflora; receptor; steroid hormone. 

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Copyright information

© Birkhäuser Verlag Basel, 1997

Authors and Affiliations

  • L. Dinan
    • 1
  • P. Whiting
    • 1
  • S. D. Sarker
    • 1
  • R. Kasai
    • 2
  • K. Yamasaki
    • 2
  1. 1.Department of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter, Devon, EX4 4QG (United Kingdom), Fax +44 1392 264 668, e-mail: l.n.dinan@exeter.ac.ukUK
  2. 2.Institute of Pharmaceutical Sciences, Hiroshima University, School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734 (Japan)JP

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